ArticleHaematologica2015
A competitive enzyme-linked immunosorbent assay specific for murine hepcidin-1: correlation with hepatic mRNA expression in established and novel models of dysregulated iron homeostasis.
Article in Haematologica, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 29 citations in OpenAlex.
- The neogenin-hemojuvelin axis in murine liver is crucial for hepcidin expression.American journal of physiology. Gastrointestinal and liver physiology · 2026Article
- In vivo silencing of intestinal DMT1 mitigates iron loading in β-thalassemia intermedia (Hbbth3/+) mice.Blood advances · 2024Article
- Malaria blood stage infection suppresses liver stage infection via host-induced interferons but not hepcidin.Nature communications · 2024Article
- Induction of Hepcidin Expression in the Renal Cortex of Sickle Cell Disease Mice.International journal of molecular sciences · 2023Article
- Aging is associated with increased brain iron through cortex-derived hepcidin expression.eLife · 2022Article
- Article
- Hepcidin-mediated Iron Regulation in P19 Cells is Detectable by Magnetic Resonance Imaging.Scientific reports · 2020Article
- Correlation Analysis Between Preoperative Serum Iron Level and Prognosis as Well as Recurrence of HCC After Radical Resection.Cancer management and research · 2020Article
- Liver iron sensing and body iron homeostasis.Blood · 2019Review
- The Functional Versatility of Transferrin Receptor 2 and Its Therapeutic Value.Pharmaceuticals (Basel, Switzerland) · 2018Review
- Matriptase-2 suppresses hepcidin expression by cleaving multiple components of the hepcidin induction pathway.The Journal of biological chemistry · 2017Article
- Hydrophilic Polyelectrolyte Multilayers Improve the ELISA System: Antibody Enrichment and Blocking Free.Polymers · 2017Article
- Modelling Systemic Iron Regulation during Dietary Iron Overload and Acute Inflammation: Role of Hepcidin-Independent Mechanisms.PLoS computational biology · 2017Article
- Efficacy estimation of erythropoiesis-stimulating agents using erythropoietin-deficient anemic mice.Haematologica · 2016Article
- Neogenin Facilitates the Induction of Hepcidin Expression by Hemojuvelin in the Liver.The Journal of biological chemistry · 2016Article
- Increased hepcidin in transferrin-treated thalassemic mice correlates with increased liver BMP2 expression and decreased hepatocyte ERK activation.Haematologica · 2016Article
- Intestine-specific Disruption of Hypoxia-inducible Factor (HIF)-2α Improves Anemia in Sickle Cell Disease.The Journal of biological chemistry · 2015Article
- Regulation of Iron Metabolism by Hepcidin under Conditions of Inflammation.The Journal of biological chemistry · 2015Review
- Combination therapy with a Tmprss6 RNAi-therapeutic and the oral iron chelator deferiprone additively diminishes secondary iron overload in a mouse model of β-thalassemia intermedia.American journal of hematology · 2015Article
- Article
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Authors and funding
12 authors at 5 institutions in 1 country.
Funding
Abstract
Mice have been essential for distinguishing the role of hepcidin in iron homeostasis. Currently, investigators monitor levels of murine hepatic hepcidin-1 mRNA as a surrogate marker for the bioactive hepcidin protein itself. Here, we describe and validate a competitive, enzyme-linked immunosorbent assay that quantifies hepcidin-1 in mouse serum and urine. The assay exhibits a biologically relevant lower limit of detection, high precision, and excellent linearity and recovery. We also demonstrate correlation between serum and urine hepcidin-1 values and validate the competitive enzyme-linked immunosorbent assay by analyzing plasma hepcidin response of mice to physiological challenges, including iron deficiency, iron overload, acute blood loss, and inflammation. Furthermore, we analyze multiple murine genetic models of iron dysregulation, including β-thalassemia intermedia (Hbb(th3/+)), hereditary hemochromatosis (Hfe(-/-), Hjv(-/-), and Tfr2(Y245X/Y245X)), hypotransferrinemia (Trf(hpx/hpx)), heterozygous transferrin receptor 1 deficiency (Tfrc(+/-)) and iron refractory iron deficiency anemia (Tmprss6(-/-) and Tmprss6(hem8/hem8)). Novel compound iron metabolism mutants were also phenotypically characterized here for the first time. We demonstrate that serum hepcidin concentrations correlate with liver hepcidin mRNA expression, transferrin saturation and non-heme liver iron. In some circumstances, serum hepcidin-1 more accurately predicts iron parameters than hepcidin mRNA, and distinguishes smaller, statistically significant differences between experimental groups.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.