ArticleProceedings of the National Academy of Sciences of the United States of America2014
SNF2 chromatin remodeler-family proteins FRG1 and -2 are required for RNA-directed DNA methylation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 34 citations in OpenAlex.
- Gene Inactivation in Transgenic Plants-A Unique Model for Studying Epigenetic Regulation of Gene Expression.Plants (Basel, Switzerland) · 2026Review
- Methylation of mycovirus DNA is mediated by the RNAi machinery in vegetative hyphae of Fusarium graminearum.Nucleic acids research · 2025Article
- The role of DNA content in shaping chromatin architecture and gene expression.The Plant journal : for cell and molecular biology · 2025Article
- Mind the gap: Epigenetic regulation of chromatin accessibility in plants.Plant physiology · 2024Review
- Epigenetic variation in early and late flowering plants of the rubber-producing Russian dandelion Taraxacum koksaghyz provides insights into the regulation of flowering time.Scientific reports · 2024Article
- Knockout of DDM1 in Physcomitrium patens disrupts DNA methylation with a minute effect on transposon regulation and development.PloS one · 2023Article
- Comparative DNA Methylome of Phytoplasma Associated Retrograde Metamorphosis in Sesame (Biology · 2022Article
- Arabidopsis CHROMATIN REMODELING 19 acts as a transcriptional repressor and contributes to plant pathogen resistance.The Plant cell · 2022Article
- Arabidopsis MORC proteins function in the efficient establishment of RNA directed DNA methylation.Nature communications · 2021Article
- Immunity onset alters plant chromatin and utilizes EDA16 to regulate oxidative homeostasis.PLoS pathogens · 2021Article
- DNA methylation-linked chromatin accessibility affects genomic architecture inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Gene Function Rather than Reproductive Mode Drives the Evolution of RNA Helicases in Sexual and Apomictic Boechera.Genome biology and evolution · 2020Article
- Diversity of Fungal DNA Methyltransferases and Their Association With DNA Methylation Patterns.Frontiers in microbiology · 2020Review
- Chromatin Remodeling and Epigenetic Regulation in Plant DNA Damage Repair.International journal of molecular sciences · 2019Review
- Arabidopsis RNA Polymerase V Mediates Enhanced Compaction and Silencing of Geminivirus and Transposon Chromatin during Host Recovery from Infection.Journal of virology · 2018Article
- RNA-directed DNA methylation involves co-transcriptional small-RNA-guided slicing of polymerase V transcripts in Arabidopsis.Nature plants · 2018Article
- The developmental regulator PKL is required to maintain correct DNA methylation patterns at RNA-directed DNA methylation loci.Genome biology · 2017Article
- Genomic and Phenomic Screens for Flower Related RING Type Ubiquitin E3 Ligases inFrontiers in plant science · 2017Article
- DNA methylation changes facilitated evolution of genes derived from Mutator-like transposable elements.Genome biology · 2016Article
- Two Components of the RNA-Directed DNA Methylation Pathway Associate with MORC6 and Silence Loci Targeted by MORC6 in Arabidopsis.PLoS genetics · 2016Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 3 countries.
Funding
Abstract
DNA methylation in Arabidopsis thaliana is maintained by at least four different enzymes: DNA methyltransferase1 (MET1), chromomethylase3 (CMT3), domains rearranged methyltransferase2 (DRM2), and chromomethylase2 (CMT2). However, DNA methylation is established exclusively by the enzyme DRM2, which acts in the RNA-directed DNA methylation (RdDM) pathway. Some RdDM components belong to gene families and have partially redundant functions, such as the endoribonucleases dicer-like 2, 3, and 4, and involved in de novo2 (IDN2) interactors IDN2-like 1 and 2. Traditional mutagenesis screens usually fail to detect genes if they are redundant, as the loss of one gene can be compensated by a related gene. In an effort to circumvent this issue, we used coexpression data to identify closely related genes that are coregulated with genes in the RdDM pathway. Here we report the discovery of two redundant proteins, SNF2-ring-helicase-like1 and -2 (FRG1 and -2) that are putative chromatin modifiers belonging to the SNF2 family of helicase-like proteins. Analysis of genome-wide bisulfite sequencing shows that simultaneous mutations of FRG1 and -2 cause defects in methylation at specific RdDM targeted loci. We also show that FRG1 physically associates with Su(var)3-9-related SUVR2, a known RdDM component, in vivo. Combined, our results identify FRG1 and FRG2 as previously unidentified components of the RdDM machinery.
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