ArticleBMC plant biology2014
Transcriptome and proteome profiling of adventitious root development in hybrid larch (Larix kaempferi × Larix olgensis).
Article in BMC plant biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed.
- Comprehensive Proteome and Acetylome Analysis of Needle Senescence inInternational journal of molecular sciences · 2024Article
- Uncovering early transcriptional regulation during adventitious root formation in Medicago sativa.BMC plant biology · 2023Article
- Analysis of oxidase activity and transcriptomic changes related to cutting propagation of hybrid larch.Scientific reports · 2023Article
- Anatomical observation and transcriptome analysis of buds reveal the association between the AP2 gene family and reproductive induction in hybrid larch (Larix kaempferi × Larix olgensis).Tree physiology · 2023Article
- Concerted control of the LaRAV1-LaCDKB1;3 module by temperature during dormancy release and reactivation of larch.Tree physiology · 2021Article
- Comparative transcriptomic analysis uncovers conserved pathways involved in adventitious root formation in poplar.Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Transcriptome-Wide Analyses Identify Dominant as the Predominantly Non-Conservative Alternative Splicing Inheritance Patterns in F1 Chickens.Frontiers in genetics · 2021Article
- Differential gene expression reveals mechanisms related to habitat divergence between hybridizing orchids from the Neotropical coastal plains.BMC plant biology · 2020Article
- Effects of parental genetic divergence on gene expression patterns in interspecific hybrids of Camellia.BMC genomics · 2019Article
- Transcriptome dynamics of rooting zone and aboveground parts of cuttings during adventitious root formation in Cryptomeria japonica D. Don.BMC plant biology · 2018Article
- Molecular Dissection of the Regenerative Capacity of Forest Tree Species: Special Focus on Conifers.Frontiers in plant science · 2018Review
- Transcriptomic Response to Nitric Oxide Treatment in Larix olgensis Henry.International journal of molecular sciences · 2015Article
- Gene expression profiling during adventitious root formation in carnation stem cuttings.BMC genomics · 2015Article
- Development and characterization of polymorphic genic-SSR markers in Larix kaempferi.Molecules (Basel, Switzerland) · 2015Article
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5 authors.
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Abstract
backgroundHybrids of larch (Larix kaempferi × Larix olgensis) are important afforestation species in northeastern China. They are routinely propagated via rooted stem cuttings. Despite the importance of rooting, little is known about the regulation of adventitious root development in larch hybrids. 454 GS FLX Titanium technology represents a new method for characterizing the transcriptomes of non-model species. This method can be used to identify differentially expressed genes, and then two-dimensional difference gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF/TOF MS) analyses can be used to analyze their corresponding proteins. In this study, we analyzed semi-lignified cuttings of two clones of L. kaempferi × L. olgensis with different rooting capacities to study the molecular basis of adventitious root development.
resultsWe analyzed two clones; clone 25-5, with strong rooting capacity, and clone 23-12, with weak rooting capacity. We constructed four cDNA libraries from 25-5 and 23-12 at two development stages. Sequencing was conducted using the 454 pyrosequencing platform. A total of 957832 raw reads was produced; 95.07% were high-quality reads, and were assembled into 45137 contigs and 61647 singletons. The functions of the unigenes, as indicated by their Gene Ontology annotation, included diverse roles in the molecular functions, biological processes, and cellular component categories. We analyzed 75 protein spots (-fold change ≥ 2, P ≤ 0.05) by 2D-DIGE, and identified the differentially expressed proteins using MALDI-TOF/TOF MS. A joint analysis of transcriptome and proteome showed genes related to two pathways, polyamine synthesis and stress response, might play an important role on adventitious root development.
conclusionsThese results provide fundamental and important information for research on the molecular mechanism of adventitious root development. We also demonstrated for the first time the combined use of two important technologies as a powerful approach to advance research on non-model, but otherwise important, larch species.
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