ArticleHuman vaccines & immunotherapeutics2014
Effect of cryopreservation of peripheral blood mononuclear cells (PBMCs) on the variability of an antigen-specific memory B cell ELISpot.
Article in Human vaccines & immunotherapeutics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
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- Parallel recovery of chromatin accessibility and gene expression dynamics from frozen human regulatory T cells.Scientific reports · 2023Article
- Article
- Quantitative and Functional Assessment of the Influence of Routinely Used Cryopreservation Media on Mononuclear Leukocytes for Medical Research.International journal of molecular sciences · 2022Article
- Antibody avidity, persistence, and response to antigen recall: comparison of vaccine adjuvants.NPJ vaccines · 2021Article
- Proteome-Wide Zika Virus CD4 T Cell Epitope and HLA Restriction Determination.ImmunoHorizons · 2020Article
- Cryopreservation of primary B cells minimally influences their signaling responses.Scientific reports · 2018Article
- Novel Method Enabling the Use of Cryopreserved Primary Acute Myeloid Leukemia Cells in Functional Drug Screens.Journal of pediatric hematology/oncology · 2017Article
- Standardized and flexible eight colour flow cytometry panels harmonized between different laboratories to study human NK cell phenotype and function.Scientific reports · 2017Article
- Optimizing recovery of frozen human peripheral blood mononuclear cells for flow cytometry.PloS one · 2017Article
- The Antibody-Secreting Cell Response to Infection: Kinetics and Clinical Applications.Frontiers in immunology · 2017Review
- Viability and Functionality of Cryopreserved Peripheral Blood Mononuclear Cells in Pediatric Dengue.Clinical and vaccine immunology : CVI · 2016Article
- Article
- Circulating B-lymphocytes as potential biomarkers of tuberculosis infection activity.PloS one · 2014Article
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8 authors.
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Abstract
The ELISpot assay is used in vaccine studies for the quantification of antigen-specific memory B cells (B(MEM)), and can be performed using cryopreserved samples. The effects of cryopreservation on B(MEM) detection and the consistency of cultured ELISpot assays when performed by different operators or laboratories are unknown. In this study, blood was taken from healthy volunteers, and a cultured ELISpot assay was used to count B(MEM) specific for 2 routine vaccine antigens (diphtheria and tetanus toxoid). Results were assessed for intra- and inter-operator variation, and the effects of cryopreservation. Cryopreserved samples were shipped to a second laboratory in order to assess inter-laboratory variation. B(MEM) frequencies were very strongly correlated when comparing fresh and frozen samples processed by the same operator, and were also very strongly correlated when comparing 2 operators in the same laboratory. Results were slightly less consistent when samples were processed in different laboratories but correlation between the 2 measurements was still very strong. Although cell viability was reduced in some cryopreserved samples due to higher temperatures during transportation, B(MEM) could still be quantified. These results demonstrate the reproducibility of the ELISpot assay across operators and laboratories, and support the use of cryopreserved samples in future B(MEM) studies.
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