ArticleMolecular microbiology2015
Discrete and overlapping functions of peptidoglycan synthases in growth, cell division and virulence of Listeria monocytogenes.
Article in Molecular microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 41 citations in OpenAlex.
- On the role of cell chaining in the attenuation of aVirulence · 2026Article
- AEmerging microbes & infections · 2026Article
- Transcriptome analysis suggests the cell wall as the primary target of the antilisterial effect of carrots.Microbiology spectrum · 2026Article
- C-di-AMP accumulation disrupts glutathione metabolism inInfection and immunity · 2024Article
- A link between STK signalling and capsular polysaccharide synthesis in Streptococcus suis.Nature communications · 2023Article
- Imbalance of peptidoglycan biosynthesis alters the cell surface charge ofCell surface (Amsterdam, Netherlands) · 2022Article
- MurA escape mutations uncouple peptidoglycan biosynthesis from PrkA signaling.PLoS pathogens · 2022Article
- EslB Is Required for Cell Wall Biosynthesis and Modification in Listeria monocytogenes.Journal of bacteriology · 2021Article
- Population structure-guided profiling of antibiotic resistance patterns in clinicalEmerging microbes & infections · 2020Article
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- PadR-type repressors controlling production of a non-canonical FtsW/RodA homologue and other trans-membrane proteins.Scientific reports · 2019Article
- The cell cycle regulator GpsB functions as cytosolic adaptor for multiple cell wall enzymes.Nature communications · 2019Article
- Suppressor Mutations Linking gpsB with the First Committed Step of Peptidoglycan Biosynthesis in Listeria monocytogenes.Journal of bacteriology · 2017Article
- Transcriptomic Analysis of the Adaptation ofFrontiers in microbiology · 2017Article
- Penicillin-Binding Protein 3 Is Essential for Growth of Pseudomonas aeruginosa.Antimicrobial agents and chemotherapy · 2017Article
- Phenotypes Associated with the Essential Diadenylate Cyclase CdaA and Its Potential Regulator CdaR in the Human Pathogen Listeria monocytogenes.Journal of bacteriology · 2016Article
- The Absence of a Mature Cell Wall Sacculus in Stable Listeria monocytogenes L-Form Cells Is Independent of Peptidoglycan Synthesis.PloS one · 2016Article
- Phosphorylation of the Peptidoglycan Synthase PonA1 Governs the Rate of Polar Elongation in Mycobacteria.PLoS pathogens · 2015Article
- The PadR-like transcriptional regulator LftR ensures efficient invasion of Listeria monocytogenes into human host cells.Frontiers in microbiology · 2015Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Upon ingestion of contaminated food, Listeria monocytogenes can cause serious infections in humans that are normally treated with β-lactam antibiotics. These target Listeria's five high molecular weight penicillin-binding proteins (HMW PBPs), which are required for peptidoglycan biosynthesis. The two bi-functional class A HMW PBPs PBP A1 and PBP A2 have transglycosylase and transpeptidase domains catalyzing glycan chain polymerization and peptide cross-linking, respectively, whereas the three class B HMW PBPs B1, B2 and B3 are monofunctional transpeptidases. The precise roles of these PBPs in the cell cycle are unknown. Here we show that green fluorescent protein (GFP)-PBP fusions localized either at the septum, the lateral wall or both, suggesting distinct and overlapping functions. Genetic data confirmed this view: PBP A1 and PBP A2 could not be inactivated simultaneously, and a conditional double mutant strain is largely inducer dependent. PBP B1 is required for rod-shape and PBP B2 for cross-wall biosynthesis and viability, whereas PBP B3 is dispensable for growth and cell division. PBP B1 depletion dramatically increased β-lactam susceptibilities and stimulated spontaneous autolysis but had no effect on peptidoglycan cross-linkage. Our in vitro virulence assays indicated that the complete set of all HMW PBPs is required for maximal virulence.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.