ArticleJournal of nanobiotechnology2014
Comparative lung toxicity of engineered nanomaterials utilizing in vitro, ex vivo and in vivo approaches.
Article in Journal of nanobiotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT07506850 (Efficacy of Casein Phosphopeptide-Amorphous Calcium Phosphate Associated With Sodium Fluoride or Stannous Fluoride in Reducing Tooth Sensitivity After In-office Dental Bleaching), which is not on this map. Cited by 12 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Efficacy of Casein Phosphopeptide-Amorphous Calcium Phosphate Associated With Sodium Fluoride or Stannous Fluoride in Reducing Tooth Sensitivity After In-office Dental Bleaching: a Randomized Clinical Trial.
Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it.
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- Establishing relationships between particle-induced in vitro and in vivo inflammation endpoints to better extrapolate between in vitro markers and in vivo fibrosis.Particle and fibre toxicology · 2023Article
- Sublethal pulmonary toxicity screening of silica nanoparticles in rats after direct intratracheal instillation.Toxicological research · 2022Article
- Approach towardEnvironmental science & technology · 2022Article
- Wood smoke particle exposure in mice reduces the severity of influenza infection.Toxicology and applied pharmacology · 2021Article
- Cell-based assays that predict in vivo neurotoxicity of urban ambient nano-sized particulate matter.Free radical biology & medicine · 2019Article
- Predicting the in vivo pulmonary toxicity induced by acute exposure to poorly soluble nanomaterials by using advanced in vitro methods.Particle and fibre toxicology · 2018Article
- Mutagenicity and Lung Toxicity of Smoldering vs. Flaming Emissions from Various Biomass Fuels: Implications for Health Effects from Wildland Fires.Environmental health perspectives · 2018Article
- Amine modification of nonporous silica nanoparticles reduces inflammatory response following intratracheal instillation in murine lungs.Toxicology letters · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
backgroundAlthough engineered nanomaterials (ENM) are currently regulated either in the context of a new chemical, or as a new use of an existing chemical, hazard assessment is still to a large extent reliant on information from historical toxicity studies of the parent compound, and may not take into account special properties related to the small size and high surface area of ENM. While it is important to properly screen and predict the potential toxicity of ENM, there is also concern that current toxicity tests will require even heavier use of experimental animals, and reliable alternatives should be developed and validated. Here we assessed the comparative respiratory toxicity of ENM in three different methods which employed in vivo, in vitro and ex vivo toxicity testing approaches.
methodsToxicity of five ENM (SiO2 (10), CeO2 (23), CeO2 (88), TiO2 (10), and TiO2 (200); parentheses indicate average ENM diameter in nm) were tested in this study. CD-1 mice were exposed to the ENM by oropharyngeal aspiration at a dose of 100 μg. Mouse lung tissue slices and alveolar macrophages were also exposed to the ENM at concentrations of 22-132 and 3.1-100 μg/mL, respectively. Biomarkers of lung injury and inflammation were assessed at 4 and/or 24 hr post-exposure.
resultsSmall-sized ENM (SiO2 (10), CeO2 (23), but not TiO2 (10)) significantly elicited pro-inflammatory responses in mice (in vivo), suggesting that the observed toxicity in the lungs was dependent on size and chemical composition. Similarly, SiO2 (10) and/or CeO2 (23) were also more toxic in the lung tissue slices (ex vivo) and alveolar macrophages (in vitro) compared to other ENM. A similar pattern of inflammatory response (e.g., interleukin-6) was observed in both ex vivo and in vitro when a dose metric based on cell surface area (μg/cm(2)), but not culture medium volume (μg/mL) was employed.
conclusionExposure to ENM induced acute lung inflammatory effects in a size- and chemical composition-dependent manner. The cell culture and lung slice techniques provided similar profiles of effect and help bridge the gap in our understanding of in vivo, ex vivo, and in vitro toxicity outcomes.
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