Evidence map›Paper›PMID 25424000›Full record

ArticleMedical science monitor : international medical journal of experimental and clinical research2014

Urethral reconstruction with tissue-engineered human amniotic scaffold in rabbit urethral injury models.

Fuli Wang, Tao Liu, Lijun Yang, Geng Zhang, Heliang Liu, Xiaomin Yi, Xiaojian Yang, Tzu-yin Lin, Weijun Qin, Jianlin Yuan

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Medical science monitor : international medical journal of experimental and clinical research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed, 3 pooled it
2.9field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 3 syntheses or guidelines pooled it, 39 citations in OpenAlex.

  1. Pooled it
  2. Tissue engineering of urethra: Systematic review of recent literature.Experimental biology and medicine (Maywood, N.J.) · 2017
    Pooled it
  3. A systematic review of animal and clinical studies on the use of scaffolds for urethral repair.Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban · 2016
    Pooled it
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  17. Electrospinning: Application and Prospects for Urologic Tissue Engineering.Frontiers in bioengineering and biotechnology · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Fuli WangDepartment of Urology, Xijing Hospital, Fourth Military Medical University, Xi'an, China (mainland).
Tao LiuDepartment of Biomedical Engineering, Fourth Military Medical University, Xi'an, China (mainland).
Lijun YangDepartment of Urology, Xijing Hospital, Fourth Military Medical University, Xi'an, China (mainland).
Geng ZhangDepartment of Urology, Xijing Hospital, Fourth Military Medical University, Xi'an, China (mainland).
Heliang LiuDepartment of Urology, Xijing Hospital, Fourth Military Medical University, Xi'an, China (mainland).
Xiaomin YiDepartment of Urology, Xijing Hospital, Fourth Military Medical University, Xi'an, China (mainland).
Xiaojian YangDepartment of Urology, Xijing Hospital, Fourth Military Medical University, Xi'an, China (mainland).
Tzu-yin LinDepartment of Internal Medicine, University of California - Davis, Sacramento, USA.
Weijun QinDepartment of Urology, Xijing Hospital, Fourth Military Medical University, Xi'an, China (mainland).
Jianlin YuanDepartment of Urology, Xijing Hospital, Fourth Military Medical University, Xi'an, China (mainland).
Xijing Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMitigating urethral injury remains a great challenge for urologists due to lack of ideal biomaterials for urethroplasty. The application of amniotic membranes (AM) over other synthetic materials make it a better potential source for urethral reconstruction. We separated the basement layer of AM to obtain denuded human amniotic scaffold (dHAS) and then inoculated primary rabbit urethral epithelial cells on the surface of dHAS to define whether this strategy minimize potential rejection and maximize the biocompatibility of human AM. MATERIAL/

methodsAfter the successful acquisition of dHAS from AM, cell-seeded dHAS were prepared and characterized. Both cell-seeded dHAS and acellular dHAS were subcutaneously implanted. Immune responses were compared by histological evaluation and CD4 cell and CD8 cell infiltrations. Then they were applied as urethroplastic materials in the rabbit models of urethral injury to fully explore the feasibility and efficacy of tissue-engineered dHAS xenografts in urethral substitution application.

resultsMild inflammatory infiltration was observed in cell-seeded dHAS grafts, as revealed by fewer accumulations of CD4 cells and CD8 cells (or neutrophils or other immune cells). Urethral defects of rabbits in the urethroplastic group with dHAS implantation (n=6) were completely resolved in one month, while there were one infection and one fistula in the control group with acellular dHAS patches (n=6). Histopathological analysis revealed mild immune response in cell-seeded dHAS group (P<0.05).

conclusionsTissue-engineered dHAS minimize potential rejection and maximize the biocompatibility of AM, which makes it a potential ideal xenograft for urethral reconstruction.

Indexed as

AmnionAnimalsCoculture TechniquesDisease Models, AnimalEpithelial CellsHumansImmunohistochemistryMalePlastic Surgery ProceduresRabbitsTissue EngineeringTissue ScaffoldsUrethra

Identifiers

PMID25424000
PMCPMC4257484
OpenAlexW2072709934

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.