ArticleThe Analyst2015
A microfluidic interface for the culture and sampling of adiponectin from primary adipocytes.
Article in The Analyst, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed.
- Optimization and validation of a fat-on-a-chip model for non-invasive therapeutic drug discovery.Frontiers in bioengineering and biotechnology · 2024Article
- Repurposing Decellularized Lung to Generate Vascularized Fat.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Review
- Single-cell transcriptome dataset of human and mouse in vitro adipogenesis models.Scientific data · 2023Article
- Increasing insulin measurement throughput by fluorescence anisotropy imaging immunoassays.Analytica chimica acta · 2022Article
- Autologous Human Immunocompetent White Adipose Tissue-on-Chip.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- The Inflammatory Profile of Obesity and the Role on Pulmonary Bacterial and Viral Infections.International journal of molecular sciences · 2021Review
- Toward improvedAPL bioengineering · 2021Article
- Towards an Insulin Resistant Adipose Model on a Chip.Cellular and molecular bioengineering · 2021Article
- A 3D human adipose tissue model within a microfluidic device.Lab on a chip · 2021Article
- Studying Adipose Tissue in the Breast Tumor Microenvironment In Vitro: Progress and Opportunities.Tissue engineering and regenerative medicine · 2020Review
- Fat-On-A-Chip Models for Research and Discovery in Obesity and Its Metabolic Comorbidities.Tissue engineering. Part B, Reviews · 2020Review
- Rapid lipolytic oscillations in ex vivo adipose tissue explants revealed through microfluidic droplet sampling at high temporal resolution.Lab on a chip · 2020Article
- WAT-on-a-chip integrating human mature white adipocytes for mechanistic research and pharmaceutical applications.Scientific reports · 2020Article
- Automated microfluidic droplet sampling with integrated, mix-and-read immunoassays to resolve endocrine tissue secretion dynamics.Lab on a chip · 2018Article
- Microfluidic systems for studying dynamic function of adipocytes and adipose tissue.Analytical and bioanalytical chemistry · 2018Review
- Automated Microfluidic Droplet-Based Sample Chopper for Detection of Small Fluorescence Differences Using Lock-In Analysis.Analytical chemistry · 2017Article
- WAT-on-a-chip: a physiologically relevant microfluidic system incorporating white adipose tissue.Lab on a chip · 2017Article
- 3D-templated, fully automated microfluidic input/output multiplexer for endocrine tissue culture and secretion sampling.Lab on a chip · 2017Article
- Monitoring cell secretions on microfluidic chips using solid-phase extraction with mass spectrometry.Analytical and bioanalytical chemistry · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
Secreted from adipose tissue, adiponectin is a vital endocrine hormone that acts in glucose metabolism, thereby establishing its crucial role in diabetes, obesity, and other metabolic disease states. Insulin exposure to primary adipocytes cultured in static conditions has been shown to stimulate adiponectin secretion. However, conventional, static methodology for culturing and stimulating adipocytes falls short of truly mimicking physiological environments. Along with decreases in experimental costs and sample volume, and increased temporal resolution, microfluidic platforms permit small-volume flowing cell culture systems, which more accurately represent the constant flow conditions through vasculature in vivo. Here, we have integrated a customized primary tissue culture reservoir into a passively operated microfluidic device made of polydimethylsiloxane (PDMS). Fabrication of the reservoir was accomplished through unique PDMS "landscaping" above sampling channels, with a design strategy targeted to primary adipocytes to overcome issues of positive cell buoyancy. This reservoir allowed three-dimensional culture of primary murine adipocytes, accurate control over stimulants via constant perfusion, and sampling of adipokine secretion during various treatments. As the first report of primary adipocyte culture and sampling within microfluidic systems, this work sets the stage for future studies in adipokine secretion dynamics.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.