ArticlePloS one2014
Mature miRNAs form secondary structure, which suggests their function beyond RISC.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 68 citations in OpenAlex.
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- Hypoxia Regulates Brown Adipocyte Differentiation and Stimulates miR-210 by HIF-1α.International journal of molecular sciences · 2024Article
- SMTRI: A deep learning-based web service for predicting small molecules that target miRNA-mRNA interactions.Molecular therapy. Nucleic acids · 2024Article
- Review
- In silico and in vitro analysis of the impact of single substitutions within EXO-motifs on Hsa-MiR-1246 intercellular transfer in breast cancer cell.Journal of applied genetics · 2023Article
- Non-canonical features of microRNAs: paradigms emerging from cardiovascular disease.Nature reviews. Cardiology · 2022Review
- Cx43-mediated sorting of miRNAs into extracellular vesicles.EMBO reports · 2022Article
- Bulge-Forming miRNases Cleave Oncogenic miRNAs at the Central Loop Region in a Sequence-Specific Manner.International journal of molecular sciences · 2022Article
- Rational design of self-assembled RNA nanostructures for HIV-1 virus assembly blockade.Nucleic acids research · 2022Article
- Identification of extremely GC-rich micro RNAs for RT-qPCR data normalization in human plasma.Frontiers in genetics · 2022Article
- Modes of action and diagnostic value of miRNAs in sepsis.Frontiers in immunology · 2022Review
- MicroRNA-100-5p and microRNA-298-5p released from apoptotic cortical neurons are endogenous Toll-like receptor 7/8 ligands that contribute to neurodegeneration.Molecular neurodegeneration · 2021Article
- Structure-aware machine learning identifies microRNAs operating as Toll-like receptor 7/8 ligands.RNA biology · 2021Article
- Pre-concentration of microRNAs by LNA-modified magnetic beads for enhancement of electrochemical detection.Scientific reports · 2021Article
- A genome-wide microRNA screen identifies the microRNA-183/96/182 cluster as a modulator of circadian rhythms.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Role of microRNAs in the predisposition to gastrointestinal malignancies.World journal of clinical cases · 2020Review
- siRNA Design to Silence the 3'UTR Region of Zika Virus.BioMed research international · 2020Article
- Genetic robustness of let-7 miRNA sequence-structure pairs.RNA (New York, N.Y.) · 2019Article
- MicroRNAs hsa-miR-495-3p and hsa-miR-486-5p suppress basal and rifampicin-induced expression of human sulfotransferase 2A1 (SULT2A1) by facilitating mRNA degradation.Biochemical pharmacology · 2019Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The generally accepted model of the miRNA-guided RNA down-regulation suggests that mature miRNA targets mRNA in a nucleotide sequence-specific manner. However, we have shown that the nucleotide sequence of miRNA is not the only determinant of miRNA specificity. Using specific nucleases, T1, V1 and S1 as well as NMR, UV/Vis and CD spectroscopies, we found that miR-21, miR-93 and miR-296 can adopt hairpin and/or homoduplex structures. The secondary structure of those miRNAs in solution is a function of RNA concentration and ionic conditions. Additionally, we have shown that a formation of miRNA hairpin is facilitated by cellular environment.Looking for functional consequences of this observation, we have perceived that structure of these miRNAs resemble RNA aptamers, short oligonucleotides forming a stable 3D structures with a high affinity and specificity for their targets. We compared structures of anti-tenascin C (anti-Tn-C) aptamers, which inhibit brain tumor glioblastoma multiforme (GBM, WHO IV) and selected miRNA. A strong overexpression of miR-21, miR-93 as well Tn-C in GBM may imply some connections between them. The structural similarity of these miRNA hairpins and anti-Tn-C aptamers indicates that miRNAs may function also beyond RISC and are even more sophisticated regulators, that it was previously expected. We think that the knowledge of the miRNA structure may give a new insight into miRNA-dependent gene regulation mechanism and be a step forward in the understanding their function and involvement in cancerogenesis. This may improve design process of anti-miRNA therapeutics.
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