Evidence map›Paper›PMID 25422748›Full record

ArticleIranian journal of basic medical sciences2014

A vector-based system for the differentiation of mouse embryonic stem cells toward germ-line cells.

Reza Ebrahimzadeh-Vesal, Mohammad Ali Shokrgozar, Karim Nayernia, Ladan Teimoori-Toolabi, Mohammad Miryounesi, Seyedmehdi Nourashrafeddin, Najmeh Ranji, Mohammad Hosein Modarressi

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Article in Iranian journal of basic medical sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 6 institutions in 3 countries.

Reza Ebrahimzadeh-VesalDepartment of Medical Genetics, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Mohammad Ali ShokrgozarNational Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran.
Karim NayerniaInstitute of Human Genetics, North East England, Stem Cell Institute, International Center for Life, Newcastle University, Newcastle, UK.
Ladan Teimoori-ToolabiMolecular Medicine Department, Biotechnology Research Center, Pasteur Institute of Iran.
Mohammad MiryounesiGenomic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Seyedmehdi NourashrafeddinMagee-Womens Research Institute & Foundation, University of Pittsburgh Medical Sciences, Pittsburgh, PA 15213, USA.
Najmeh RanjiDepartment of Genetics, Faculty of Sciences, Islamic Azad University, Rasht Branch, Rasht, Iran.
Mohammad Hosein ModarressiDepartment of Medical Genetics, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Pasteur Institute of Iran · IRTehran University of Medical Sciences · IRIslamic Azad University Rasht Branch · IRNewcastle University · GBShahid Beheshti University of Medical Sciences · IRUniversity of Pittsburgh · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesTo culture the in vitro mouse embryonic stem cells (mESCs) and to direct their differentiation to germ-line cells; in present study we used a vector backbone containing the fusion construct Stra8-EGFP to select differentiated ES cells that entered meiosis. Retinoic acid was used to differentiate embryonic stem cells to germ cells. MATERIALS AND

methodsA fragment of Stra8 gene promoter (-1400 to +7) was inserted in ScaI/HindIII multiple cloning site of pEGFP-1 vector. The electroporation was done on embryonic stem cells and positive colonies were selected as puromycin-resistant after three weeks of treatment with puromycin. All-trans retinoic acid (RA) was used for differentiation of mESCs at final concentration of 10(-)5M. The expression of protamine 1 (Prm1) gene was checked as post meiotic marker in differentiated mESCs after 5, 10, 15, 21 and 30 days after RA induction.

resultsThe PCR amplification by specific primers for Stra8-EGFP fusion gene was detected in DNA sample from mESCs after electroporation and puromycin treatment. GFP-positive mESC colonies were observed after 72 hr RA induction. The protamine 1 gene was expressed after 21 days of RA induction.

conclusionIn this study, we demonstrated the in vitro generation of mouse embryonic stem cells to germ cells by using a backbone vector containing the fusion gene Stra8-EGFP. The Stra8 gene is a retinoic acid-responsive protein and is able to regulate meiotic initiation.

Indexed as

DifferentiationGerm-line cellsMouse embryonic stem cellVector-based system

Identifiers

PMID25422748
PMCPMC4240789
OpenAlexW1606009384

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