ArticleProceedings of the National Academy of Sciences of the United States of America2014
Oscillatory AAA+ ATPase Knk1 constitutes a novel morphogenetic pathway in fission yeast.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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3 citing papers in PubMed, 8 citations in OpenAlex.
- Eng2, a new player involved in feedback loop regulation of Cdc42 activity in fission yeast.Scientific reports · 2021Article
- Cell Polarity in Yeast.Annual review of cell and developmental biology · 2017Review
- Rga6 is a Fission Yeast Rho GAP Involved in Cdc42 Regulation of Polarized Growth.Molecular biology of the cell · 2016Article
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4 authors at 1 institution in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
Cellular morphogenesis relies partly on cell polarization by the cytoskeleton. In the fission yeast Schizosaccharomyces pombe, it is well established that microtubules (MTs) deliver the spatial cue Tea1, a kelch repeat protein, to the tip regions to direct the growth machinery at the cell tips driving the linear extension of the rod-shaped organism to maintain a straight long axis. Here, we report the characterization of Knk1 (kink), a previously unidentified member of the superfamily of ATPases associated with various cellular activities (AAA(+)), whose deletion causes a unique morphological defect characterized by the formation of kinks close to cell tips. Through genetic analysis, we place Knk1 into a novel pathway controlling cell shape independently of MTs and Tea1. Knk1 localizes at cell tips. Its localization is mediated by the Knk1 N terminus and is enhanced upon ATP binding to the C-terminal ATPase domain. Furthermore, Knk1 tip recruitment is regulated by SRC-like adaptor 2 (Sla2) and cell division cycle 42 (Cdc42) independently of Sla2's role in endocytosis. Finally, we discovered that Knk1 shows an anticorrelated oscillatory behavior between the two cell tips at a periodicity that is different from the reported oscillatory Cdc42 dynamics.
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