ArticleProceedings of the National Academy of Sciences of the United States of America2014
Whole-cell, multicolor superresolution imaging using volumetric multifocus microscopy.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 111 citations in OpenAlex.
- In-depth single molecule localization microscopy using adaptive optics and single objective light-sheet microscopy.Nature communications · 2025Article
- DHPSFU: a Fiji plugin for fast and accurate double helix-PSF 3D single-molecule localisation microscopy.Scientific reports · 2025Article
- Long axial range 3D single-particle tracking using birefringent substrates.Nature communications · 2025Article
- In Operando Locally-Resolved Photophysics in Perovskite Solar Cells by Correlation Clustering Imaging.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Whole-cell multi-target single-molecule super-resolution imaging in 3D with microfluidics and a single-objective tilted light sheet.Nature communications · 2024Article
- Long-Axial-Range Double-Helix Point Spread Functions for 3D Volumetric Super-Resolution Imaging.The journal of physical chemistry. B · 2024Article
- Bi-Plane Multicolor Scanning Illumination Microscopy with Multispot Excitation and a Distorted Diffraction Grating.Biosensors · 2024Article
- Whole-cell multi-target single-molecule super-resolution imaging in 3D with microfluidics and a single-objective tilted light sheet.bioRxiv : the preprint server for biology · 2024Article
- Multifocal microscopy for functional imaging of neural systems.Neurophotonics · 2024Article
- Long axial-range double-helix point spread functions for 3D volumetric super-resolution imaging.bioRxiv : the preprint server for biology · 2024Article
- Highly sensitive volumetric single-molecule imaging.Nanophotonics (Berlin, Germany) · 2024Article
- High-fidelity 3D live-cell nanoscopy through data-driven enhanced super-resolution radial fluctuation.Nature methods · 2023Article
- Article
- Recent Advances in Single-Molecule Tracking and Imaging Techniques.Annual review of analytical chemistry (Palo Alto, Calif.) · 2023Review
- High-resolution multi-z confocal microscopy with a diffractive optical element.Biomedical optics express · 2023Article
- SPITFIR(e): a supermaneuverable algorithm for fast denoising and deconvolution of 3D fluorescence microscopy images and videos.Scientific reports · 2023Article
- Ultrastructure expansion microscopy reveals the cellular architecture of budding and fission yeast.Journal of cell science · 2022Article
- Fiber-based 3D nano-printed holography with individually phase-engineered remote points.Scientific reports · 2022Article
- Multicolor multifocal 3D microscopy using in-situ optimization of a spatial light modulator.Scientific reports · 2022Article
- Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Single molecule-based superresolution imaging has become an essential tool in modern cell biology. Because of the limited depth of field of optical imaging systems, one of the major challenges in superresolution imaging resides in capturing the 3D nanoscale morphology of the whole cell. Despite many previous attempts to extend the application of photo-activated localization microscopy (PALM) and stochastic optical reconstruction microscopy (STORM) techniques into three dimensions, effective localization depths do not typically exceed 1.2 µm. Thus, 3D imaging of whole cells (or even large organelles) still demands sequential acquisition at different axial positions and, therefore, suffers from the combined effects of out-of-focus molecule activation (increased background) and bleaching (loss of detections). Here, we present the use of multifocus microscopy for volumetric multicolor superresolution imaging. By simultaneously imaging nine different focal planes, the multifocus microscope instantaneously captures the distribution of single molecules (either fluorescent proteins or synthetic dyes) throughout an ∼ 4-µm-deep volume, with lateral and axial localization precisions of ∼ 20 and 50 nm, respectively. The capabilities of multifocus microscopy to rapidly image the 3D organization of intracellular structures are illustrated by superresolution imaging of the mammalian mitochondrial network and yeast microtubules during cell division.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.