ArticleProceedings of the National Academy of Sciences of the United States of America2014
Osteoinductivity of engineered cartilaginous templates devitalized by inducible apoptosis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 71 citations in OpenAlex.
- Engineered and decellularized human cartilage graft exhibits intrinsic immunosuppressive properties and full skeletal repair capacity.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Synthetic scaffolds functionalized with mesenchymal stem/stromal cells-derived extracellular matrix for bone tissue engineering: a review.RSC advances · 2025Review
- Advances in abiotic tissue-based biomaterials: A focus on decellularization and devitalization techniques.Materials today. Bio · 2025Article
- Application of decellularization methods for scaffold production: advantages, disadvantages, biosafety and modifications.Frontiers in bioengineering and biotechnology · 2025Review
- Unraveling devitalization: its impact on immune response and ectopic bone remodeling from autologous and allogeneic callus mimics.Stem cells translational medicine · 2024Article
- Acceleration of Bone Regeneration Induced by a Soft-Callus Mimetic Material.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Decellularization in Tissue Engineering and Regenerative Medicine: Evaluation, Modification, and Application Methods.Frontiers in bioengineering and biotechnology · 2022Review
- Is extracellular matrix (ECM) a promising scaffold biomaterial for bone repair?Histology and histopathology · 2021Review
- Manufacturing of Human Tissues as off-the-Shelf Grafts Programmed to Induce Regeneration.Advanced materials (Deerfield Beach, Fla.) · 2021Article
- A simple method for decellularizing a cell-derived matrix for bone cell cultivation and differentiation.Journal of materials science. Materials in medicine · 2021Article
- Decellularized Cartilage Extracellular Matrix Incorporated Silk Fibroin Hybrid Scaffolds for Endochondral Ossification Mediated Bone Regeneration.International journal of molecular sciences · 2021Article
- Bone Matrix Non-Collagenous Proteins in Tissue Engineering: Creating New Bone by Mimicking the Extracellular Matrix.Polymers · 2021Review
- Turning Nature's own processes into design strategies for living bone implant biomanufacturing: a decade of Developmental Engineering.Advanced drug delivery reviews · 2021Review
- Skeletal Stem Cells as the Developmental Origin of Cellular Niches for Hematopoietic Stem and Progenitor Cells.Current topics in microbiology and immunology · 2021Article
- Orthotopic Bone Formation by Streamlined Engineering and Devitalization of Human Hypertrophic Cartilage.International journal of molecular sciences · 2020Article
- Cell Bioprinting: The 3D-Bioplotter™ Case.Materials (Basel, Switzerland) · 2019Review
- Fate Distribution and Regulatory Role of Human Mesenchymal Stromal Cells in Engineered Hematopoietic Bone Organs.iScience · 2019Article
- Co-culture cell-derived extracellular matrix loaded electrospun microfibrous scaffolds for bone tissue engineering.Materials science & engineering. C, Materials for biological applications · 2019Article
- Extracellular Matrices to Modulate the Innate Immune Response and Enhance Bone Healing.Frontiers in immunology · 2019Review
- 3D Bone Biomimetic Scaffolds for Basic and Translational Studies with Mesenchymal Stem Cells.International journal of molecular sciences · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The role of cell-free extracellular matrix (ECM) in triggering tissue and organ regeneration has gained increased recognition, yet current approaches are predominantly based on the use of ECM from fully developed native tissues at nonhomologous sites. We describe a strategy to generate customized ECM, designed to activate endogenous regenerative programs by recapitulating tissue-specific developmental processes. The paradigm was exemplified in the context of the skeletal system by testing the osteoinductive capacity of engineered and devitalized hypertrophic cartilage, which is the primordial template for the development of most bones. ECM was engineered by inducing chondrogenesis of human mesenchymal stromal cells and devitalized by the implementation of a death-inducible genetic device, leading to cell apoptosis on activation and matrix protein preservation. The resulting hypertrophic cartilage ECM, tested in a stringent ectopic implantation model, efficiently remodeled to form de novo bone tissue of host origin, including mature vasculature and a hematopoietic compartment. Importantly, cartilage ECM could not generate frank bone tissue if devitalized by standard "freeze & thaw" (F&T) cycles, associated with a significant loss of glycosaminoglycans, mineral content, and ECM-bound cytokines critically involved in inflammatory, vascularization, and remodeling processes. These results support the utility of engineered ECM-based devices as off-the-shelf regenerative niches capable of recruiting and instructing resident cells toward the formation of a specific tissue.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.