Evidence map›Paper›PMID 25422367›Full record

ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2015

Protein profiling of isolated uterine AA amyloidosis causing fetal death in goats.

Patricia M Gaffney, Bradd Barr, Joan D Rowe, Cyrus Bett, Ioannis Drygiannakis, Federico Giannitti, Margarita Trejo, Majid Ghassemian, Patrice Martin, Eliezer Masliah and 1 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.0field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 15 citations in OpenAlex.

  1. Article
  2. Polymorphism of Amyloid Fibrils In Vivo.Angewandte Chemie (International ed. in English) · 2016
    Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 2 countries.

Patricia M GaffneyDepartments of *Pathology, Neuroscience, and Chemistry and Biochemistry, University of California, San Diego, La Jolla, California, USA; Departments of Pathology, Immunology and Microbiology and Population Health & Reproduction, and California Animal Health and Food Safety Laboratory System, University of California, Davis, Davis, California, USA; and INRA, UMR1313 Unité Génétique Animale et Biologie Intégrative, Jouy-en-Josas, France.
Bradd BarrDepartments of *Pathology, Neuroscience, and Chemistry and Biochemistry, University of California, San Diego, La Jolla, California, USA; Departments of Pathology, Immunology and Microbiology and Population Health & Reproduction, and California Animal Health and Food Safety Laboratory System, University of California, Davis, Davis, California, USA; and INRA, UMR1313 Unité Génétique Animale et Biologie Intégrative, Jouy-en-Josas, France.
Joan D RoweDepartments of *Pathology, Neuroscience, and Chemistry and Biochemistry, University of California, San Diego, La Jolla, California, USA; Departments of Pathology, Immunology and Microbiology and Population Health & Reproduction, and California Animal Health and Food Safety Laboratory System, University of California, Davis, Davis, California, USA; and INRA, UMR1313 Unité Génétique Animale et Biologie Intégrative, Jouy-en-Josas, France.
Cyrus BettDepartments of *Pathology, Neuroscience, and Chemistry and Biochemistry, University of California, San Diego, La Jolla, California, USA; Departments of Pathology, Immunology and Microbiology and Population Health & Reproduction, and California Animal Health and Food Safety Laboratory System, University of California, Davis, Davis, California, USA; and INRA, UMR1313 Unité Génétique Animale et Biologie Intégrative, Jouy-en-Josas, France.
Ioannis DrygiannakisDepartments of *Pathology, Neuroscience, and Chemistry and Biochemistry, University of California, San Diego, La Jolla, California, USA; Departments of Pathology, Immunology and Microbiology and Population Health & Reproduction, and California Animal Health and Food Safety Laboratory System, University of California, Davis, Davis, California, USA; and INRA, UMR1313 Unité Génétique Animale et Biologie Intégrative, Jouy-en-Josas, France.
Federico GiannittiDepartments of *Pathology, Neuroscience, and Chemistry and Biochemistry, University of California, San Diego, La Jolla, California, USA; Departments of Pathology, Immunology and Microbiology and Population Health & Reproduction, and California Animal Health and Food Safety Laboratory System, University of California, Davis, Davis, California, USA; and INRA, UMR1313 Unité Génétique Animale et Biologie Intégrative, Jouy-en-Josas, France.
Margarita TrejoDepartments of *Pathology, Neuroscience, and Chemistry and Biochemistry, University of California, San Diego, La Jolla, California, USA; Departments of Pathology, Immunology and Microbiology and Population Health & Reproduction, and California Animal Health and Food Safety Laboratory System, University of California, Davis, Davis, California, USA; and INRA, UMR1313 Unité Génétique Animale et Biologie Intégrative, Jouy-en-Josas, France.
Majid GhassemianDepartments of *Pathology, Neuroscience, and Chemistry and Biochemistry, University of California, San Diego, La Jolla, California, USA; Departments of Pathology, Immunology and Microbiology and Population Health & Reproduction, and California Animal Health and Food Safety Laboratory System, University of California, Davis, Davis, California, USA; and INRA, UMR1313 Unité Génétique Animale et Biologie Intégrative, Jouy-en-Josas, France.
Patrice MartinDepartments of *Pathology, Neuroscience, and Chemistry and Biochemistry, University of California, San Diego, La Jolla, California, USA; Departments of Pathology, Immunology and Microbiology and Population Health & Reproduction, and California Animal Health and Food Safety Laboratory System, University of California, Davis, Davis, California, USA; and INRA, UMR1313 Unité Génétique Animale et Biologie Intégrative, Jouy-en-Josas, France.
Eliezer MasliahDepartments of *Pathology, Neuroscience, and Chemistry and Biochemistry, University of California, San Diego, La Jolla, California, USA; Departments of Pathology, Immunology and Microbiology and Population Health & Reproduction, and California Animal Health and Food Safety Laboratory System, University of California, Davis, Davis, California, USA; and INRA, UMR1313 Unité Génétique Animale et Biologie Intégrative, Jouy-en-Josas, France.
Christina J SigurdsonDepartments of *Pathology, Neuroscience, and Chemistry and Biochemistry, University of California, San Diego, La Jolla, California, USA; Departments of Pathology, Immunology and Microbiology and Population Health & Reproduction, and California Animal Health and Food Safety Laboratory System, University of California, Davis, Davis, California, USA; and INRA, UMR1313 Unité Génétique Animale et Biologie Intégrative, Jouy-en-Josas, France csigurdson@ucsd.edu.
University of California, San Diego · USUniversity of California, Davis · USWest Health · USGénétique Animale et Biologie Intégrative · FRUniversity of California System · US

Funding

UCSD Research Training Program for VeterinariansT32OD017863 · OD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ERNST, PETER B., SIGURDSON, CHRISTINA · 2014 to 2023
$2.0M
NIH HHS T32 OD017863
6 · The paper itself

Abstract

Pathologic amyloid accumulates in the CNS or in peripheral organs, yet the mechanism underlying the targeting of systemic amyloid deposits is unclear. Serum amyloid A (SAA) 1 and 2 are produced predominantly by the liver and form amyloid most commonly in the spleen, liver, and kidney. In contrast, SAA3 is produced primarily extrahepatically and has no causal link to amyloid formation. Here, we identified 8 amyloidosis cases with amyloid composed of SAA3 expanding the uterine wall of goats with near-term fetuses. Uterine amyloid accumulated in the endometrium, only at the site of placental attachment, compromising maternal-fetal gas and nutrient exchange and leading to fetal ischemia and death. No other organ contained amyloid. SAA3 mRNA levels in the uterine endometrium were as high as SAA2 in the liver, yet mass spectrometry of the insoluble uterine peptides identified SAA3 as the predominant protein, and not SAA1 or SAA2. These findings suggest that high local SAA3 production led to deposition at this unusual site. Although amyloid A (AA) amyloid deposits typically consist of an N-terminal fragment of SAA1 or SAA2, here, abundant C-terminal peptides indicated that the uterine amyloid was largely composed of full-length SAA3. The exclusive deposition of SAA3 amyloid in the uterus, together with elevated uterine SAA3 transcripts, suggests that the uterine amyloid deposits were due to locally produced SAA3. This is the first report of SAA3 as a cause of amyloidosis and of AA amyloid deposited exclusively in the uterus.

Indexed as

ApoptosisFetal DeathAmino Acid SequenceAmyloidAmyloidosisAnimalsBlotting, WesternCell ProliferationCells, CulturedChromatography, LiquidFemaleGoatsImmunoenzyme TechniquesMolecular Sequence DataProteomeReal-Time Polymerase Chain ReactionAmyloidProteomeRNA, MessengerSerum Amyloid A Proteinamyloidmass spectrometryprotein misfolding

Identifiers

PMID25422367
PMCPMC6137862
OpenAlexW2095789694

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.