ArticleJournal of bacteriology2015
A small unstructured region in Vibrio cholerae ToxT mediates the response to positive and negative effectors and ToxT proteolysis.
Article in Journal of bacteriology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 26 citations in OpenAlex.
- Article
- Article
- Advances in cholera research: from molecular biology to public health initiatives.Frontiers in microbiology · 2023Review
- FoxR is an AraC-like transcriptional regulator of ferrioxamine uptake in Salmonella enterica.Molecular microbiology · 2022Article
- ToxT Regulon Is Nonessential for Vibrio cholerae Colonization in Adult Mice.Applied and environmental microbiology · 2022Article
- Mr.Vc v2: An updated version of database with increased data of transcriptome and experimental validated interactions.Frontiers in microbiology · 2022Article
- The Role of the Two-Component QseBC Signaling System in Biofilm Formation and Virulence of HypervirulentFrontiers in microbiology · 2022Article
- AraC-type regulators HilC and RtsA are directly controlled by an intestinal fatty acid to regulate Salmonella invasion.Molecular microbiology · 2021Article
- Bile Salts Promote ToxR Regulon Activation during Growth under Virulence-Inducing Conditions.Infection and immunity · 2021Article
- Backbone Interactions Between Transcriptional Activator ExsA and Anti-Activator ExsD Facilitate Regulation of the Type III Secretion System in Pseudomonas aeruginosa.Scientific reports · 2020Article
- Cholera Toxin Production inFrontiers in microbiology · 2020Article
- Regulated Proteolysis inFrontiers in cellular and infection microbiology · 2019Review
- Redox, amino acid, and fatty acid metabolism intersect with bacterial virulence in the gut.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- The Fatty Acid Regulator FadR Influences the Expression of the Virulence Cascade in the El Tor Biotype of Vibrio cholerae by Modulating the Levels of ToxT via Two Different Mechanisms.Journal of bacteriology · 2017Article
- A new class of inhibitors of the AraC family virulence regulator Vibrio cholerae ToxT.Scientific reports · 2017Article
- Staying Alive: Vibrio cholerae's Cycle of Environmental Survival, Transmission, and Dissemination.Microbiology spectrum · 2016Review
- Conjugated Linoleic Acid Reduces Cholera Toxin Production In Vitro and In Vivo by Inhibiting Vibrio cholerae ToxT Activity.Antimicrobial agents and chemotherapy · 2015Article
- Intestinal Colonization Dynamics of Vibrio cholerae.PLoS pathogens · 2015Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Vibrio cholerae is the causative agent of the severe diarrheal disease cholera. The production of the virulence factors that are required for human disease is controlled by a complex network of transcriptional and posttranscriptional regulators. ToxT is the transcription regulator that directly controls the production of the two major virulence factors, toxin-coregulated pilus (TCP) and cholera toxin (CT). The solved crystal structure of ToxT revealed an unstructured region in the N-terminal domain between residues 100 and 110. This region and the surrounding amino acids have been previously implicated in ToxT proteolysis, resistance to inhibition by negative effectors, and ToxT dimerization. To better characterize this region, site-directed mutagenesis was performed to assess the effects on ToxT proteolysis and bile sensitivity. This analysis identified specific mutations within this unstructured region that prevent ToxT proteolysis and other mutations that reduce inhibition by bile and unsaturated fatty acids. In addition, we found that mutations that affect the sensitivity of ToxT to bile also affect the sensitivity of ToxT to its positive effector, bicarbonate. These results suggest that a small unstructured region in the ToxT N-terminal domain is involved in multiple aspects of virulence gene regulation and response to human host signals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.