ArticleLab on a chip2015
Efficient single-cell poration by microsecond laser pulses.
Article in Lab on a chip, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
14 citing papers in PubMed.
- Laser-Induced Intracellular Delivery: Exploiting Gold-Coated Spiky Polymeric Nanoparticles and Gold Nanorods under Near-Infrared Pulses for Single-Cell Nano-Photon-Poration.Micromachines · 2024Article
- Three-dimensional array of microbubbles sonoporation of cells in microfluidics.Frontiers in bioengineering and biotechnology · 2024Article
- Translocation and fate of nanospheres in pheochromocytoma cells following exposure to synchrotron-sourced terahertz radiation.Journal of synchrotron radiation · 2023Article
- Continuous microfluidic flow-through protocol for selective and image-activated electroporation of single cells.RSC advances · 2023Article
- Review of Bubble Applications in Microrobotics: Propulsion, Manipulation, and Assembly.Micromachines · 2022Review
- An Overview of Cell Membrane Perforation and Resealing Mechanisms for Localized Drug Delivery.Pharmaceutics · 2022Review
- Effect of laser pulse shaping on photoacoustic dosimetry in retinal models.Biomedical optics express · 2020Article
- Advanced physical techniques for gene delivery based on membrane perforation.Drug delivery · 2018Review
- Cell Membrane Disruption by Vertical Micro-/Nanopillars: Role of Membrane Bending and Traction Forces.ACS applied materials & interfaces · 2018Article
- Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts.Chemical reviews · 2018Review
- Soft electroporation for delivering molecules into tightly adherent mammalian cells through 3D hollow nanoelectrodes.Scientific reports · 2017Article
- Cooperative Micromanipulation Using the Independent Actuation of Fifty Microrobots in Parallel.Scientific reports · 2017Article
- Localized Single-Cell Lysis and Manipulation Using Optothermally-Induced Bubbles.Micromachines · 2017Article
- Vision-assisted micromanipulation using closed-loop actuation of multiple microrobots.Robotics and biomimetics · 2017Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Payloads including FITC-Dextran dye and plasmids were delivered into NIH/3T3 fibroblasts using microbubbles produced by microsecond laser pulses to induce pores in the cell membranes. Two different operational modes were used to achieve molecular delivery. Smaller molecules, such as the FITC-Dextran dye, were delivered via a scanning-laser mode. The poration efficiency and the cell viability were both 95.1 ± 3.0%. Relatively larger GFP plasmids can be delivered efficiently via a fixed-laser mode, which is a more vigorous method that can create larger transient pores in the cell membrane. The transfection efficiency of 5.7 kb GFP plasmid DNA can reach to 86.7 ± 3.3%. Using this cell poration system, targeted single cells can be porated with high resolution, and cells can be porated in arbitrary patterns.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.