ArticleMalaria journal2014
A reliable and rapid method for molecular detection of malarial parasites using microwave irradiation and loop mediated isothermal amplification.
Article in Malaria journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 33 citations in OpenAlex.
- Validated antimalarial drug target discovery using genome-scale metabolic modeling.Antimicrobial agents and chemotherapy · 2025Article
- Comparison of Three Methods to Extract Plasmodium falciparum DNA from Whole Blood and Dried Blood Spots.The American journal of tropical medicine and hygiene · 2024Article
- Comparison of 3 DNA extraction methods for extracting DNA from an adult Culex quinquefasciatus (Diptera: Culicidae).Journal of insect science (Online) · 2023Article
- Evaluation of A Simple DNA Extraction Method and Its Combination with Loop-Mediated Isothermal Amplification Assays for RapidTropical medicine and infectious disease · 2023Article
- A simple alkali lysis method for Plasmodium falciparum DNA extraction from filter paper blood samples.Molecular and biochemical parasitology · 2023Article
- Usefulness of a commercial LAMP assay for detection of malaria infection, including Plasmodium knowlesi cases, in returning travelers in Spain.BMC research notes · 2022Article
- Noninvasive Approach ofBioMed research international · 2022Article
- Applications of Microwave Energy in Medicine.Biosensors · 2021Review
- Performance and Application of Commercially Available Loop-Mediated Isothermal Amplification (LAMP) Kits in Malaria Endemic and Non-Endemic Settings.Diagnostics (Basel, Switzerland) · 2021Review
- Advances in point-of-care nucleic acid extraction technologies for rapid diagnosis of human and plant diseases.Biosensors & bioelectronics · 2020Review
- Advances in Directly Amplifying Nucleic Acids from Complex Samples.Biosensors · 2019Review
- Poor Diagnostic Performance of a Species-Specific Loop-Mediated Isothermal Amplification (LAMP) Platform for Malaria.European journal of microbiology & immunology · 2018Article
- An Alternative Method for ExtractingThe Korean journal of parasitology · 2018Article
- Recent Progress in the Development of Diagnostic Tests for Malaria.Diagnostics (Basel, Switzerland) · 2017Review
- Development of loop-mediated isothermal amplification with Plasmodium falciparum unique genes for molecular diagnosis of human malaria.Pathogens and global health · 2017Article
- LAMP kit for diagnosis of non-falciparum malaria in Plasmodium ovale infected patients.Malaria journal · 2017Article
- Feasibility and implementation of community-based malaria case management with integrated vector control in the Democratic Republic of Congo.Malaria journal · 2016Article
- A paper and plastic device for the combined isothermal amplification and lateral flow detection of Plasmodium DNA.Malaria journal · 2015Article
- Malaria elimination in Haiti by the year 2020: an achievable goal?Malaria journal · 2015Article
- Opisthorchiasis: an overlooked danger.PLoS neglected tropical diseases · 2015Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundImproved living conditions together with appropriate diagnosis can reduce avoidable malarial deaths substantially. Microscopy remains the gold standard in the diagnosis of malaria. However, rapid molecular diagnostic tests (RmDT) are becoming increasingly important and will, most likely, be the diagnostic techniques of choice in the next years.
methodsIn this study, a rapid and reliable nucleic acid extraction procedure from human blood and malarial parasites using microwave irradiation as a promising platform is described. In addition, a tailored loop mediated isothermal amplification (LAMP) methodology that utilizes hydroxynaphthol blue (HNB) and Bst 2.0 DNA polymerases in molecular detection of malarial parasites is described.
resultsFollowing microwave irradiation for DNA isolation, conventional PCR assays were able to detect up to five malaria parasites/μl. The LAMP methodology described here was capable to detect as low as one Plasmodium falciparum parasite/μl after DNA extraction by microwave irradiation. A turnover time of 45 minutes from nucleic acid extraction to final visual read-out was achieved.
conclusionsThe described procedure offers a cheap, simple and fast method of molecular detection of malaria parasites. This test can easily be performed in basic laboratories. The methodology has been validated as a proof of concept and has specifically be developed for use at low-resource settings. Such RmDTs may aid health providers to make timely therapeutic interventions in malaria endemic regions.
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