Evidence map›Paper›PMID 25421323›Full record

ArticleMarine drugs2014

Delivery of berberine using chitosan/fucoidan-taurine conjugate nanoparticles for treatment of defective intestinal epithelial tight junction barrier.

Shao-Jung Wu, Trong-Ming Don, Cheng-Wei Lin, Fwu-Long Mi

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Marine drugs, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.

0numbers the graph read from it
0cells of the map it votes in
39citing papers in PubMed
11.0field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

39 citing papers in PubMed, 122 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Article
  5. Article
  6. Cinnamon (Food science and biotechnology · 2023
    Article
  7. Fucoidan, a brown seaweed polysaccharide in nanodrug delivery.Drug delivery and translational research · 2023
    Review
  8. Review
  9. Article
  10. Article
  11. Review
  12. Review
  13. Review
  14. Article
  15. Review
  16. Article
  17. Article
  18. Article
  19. Bioactive Carbohydrate Polymers-Between Myth and Reality.Molecules (Basel, Switzerland) · 2021
    Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 1 country.

Shao-Jung WuDepartment of Chemical Engineering, Ming Chi University of Technology, New Taipei City 243, Taiwan. sjwu@mail.mcut.edu.tw.
Trong-Ming DonDepartment of Chemical and Materials Engineering, Tamkang University, New Taipei City 251, Taiwan. tmdon@mail.tku.edu.tw.
Cheng-Wei LinDepartment of Biochemistry and Molecular Cell Biology, School of medicine, Taipei Medical University, Taipei City 110, Taiwan. cwlin@tmu.edu.tw.
Fwu-Long MiDepartment of Biochemistry and Molecular Cell Biology, School of medicine, Taipei Medical University, Taipei City 110, Taiwan. flmi530326@tmu.edu.tw.
Taipei Medical University · TWMing Chi University of Technology · TWTamkang University · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bacterial-derived lipopolysaccharides (LPS) can cause defective intestinal barrier function and play an important role in the development of inflammatory bowel disease. In this study, a nanocarrier based on chitosan and fucoidan was developed for oral delivery of berberine (Ber). A sulfonated fucoidan, fucoidan-taurine (FD-Tau) conjugate, was synthesized and characterized by Fourier transform infrared (FTIR) spectroscopy. The FD-Tau conjugate was self-assembled with berberine and chitosan (CS) to form Ber-loaded CS/FD-Tau complex nanoparticles with high drug loading efficiency. Berberine release from the nanoparticles had fast release in simulated intestinal fluid (SIF, pH 7.4), while the release was slow in simulated gastric fluid (SGF, pH 2.0). The effect of the berberine-loaded nanoparticles in protecting intestinal tight-junction barrier function against nitric oxide and inflammatory cytokines released from LPS-stimulated macrophage was evaluated by determining the transepithelial electrical resistance (TEER) and paracellular permeability of a model macromolecule fluorescein isothiocyanate-dextran (FITC-dextran) in a Caco-2 cells/RAW264.7 cells co-culture system. Inhibition of redistribution of tight junction ZO-1 protein by the nanoparticles was visualized using confocal laser scanning microscopy (CLSM). The results suggest that the nanoparticles may be useful for local delivery of berberine to ameliorate LPS-induced intestinal epithelia tight junction disruption, and that the released berberine can restore barrier function in inflammatory and injured intestinal epithelial.

Indexed as

Drug Delivery SystemsAnimalsBerberineCaco-2 CellsCell LineChitosanCoculture TechniquesCytokinesDextransDrug CarriersFluorescein-5-isothiocyanateHumansHydrogen-Ion ConcentrationIntestinal MucosaLipopolysaccharidesMiceBerberineChitosanCytokinesDextransDrug CarriersFluorescein-5-isothiocyanatefluorescein isothiocyanate dextranfucoidanLipopolysaccharidesNitric OxidePolysaccharidesTaurine

Identifiers

PMID25421323
PMCPMC4245551
OpenAlexW2063736792

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.