ArticleBMC microbiology2014
Hepatitis A virus subgenotyping based on RT-qPCR assays.
Article in BMC microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 31 citations in OpenAlex.
- Therapeutic, preventive, and pathogenetic advances in viral hepatitis.Frontiers in cellular and infection microbiology · 2026Review
- Whole-Genome Sequencing-Based Confirmatory Methods on RT-qPCR Results for the Detection of Foodborne Viruses in Frozen Berries.Food and environmental virology · 2024Article
- High-performance enrichment-based genome sequencing to support the investigation of hepatitis A virus outbreaks.Microbiology spectrum · 2024Article
- Development and Evaluation of a Molecular Hepatitis A Virus Assay for Serum and Stool Specimens.Viruses · 2022Article
- Genotyping and Molecular Diagnosis of Hepatitis A Virus in Human Clinical Samples Using Multiplex PCR-Based Next-Generation Sequencing.Microorganisms · 2022Article
- Inactivation of Hepatovirus A in wastewater by 254 nm ultraviolet-C irradiation.Environmental science and pollution research international · 2021Article
- Discriminating the eight genotypes of the porcine circovirus type 2 with TaqMan-based real-time PCR.Virology journal · 2021Article
- Occurrence of Human Enteric Viruses in Water Sources and Shellfish: A Focus on Africa.Food and environmental virology · 2021Review
- New Subgenotyping and Consensus Real-Time Reverse Transcription-PCR Assays for Hepatitis A Outbreak Surveillance.Journal of clinical microbiology · 2019Article
- Sensitive Genotyping of Foodborne-Associated Human Noroviruses and Hepatitis A Virus Using an Array-Based Platform.Sensors (Basel, Switzerland) · 2017Article
- Ethnopharmacological Approaches for Therapy of Jaundice: Part I.Frontiers in pharmacology · 2017Review
- Quantitative PCR Detection and Characterisation of Human Adenovirus, Rotavirus and Hepatitis A Virus in Discharged Effluents of Two Wastewater Treatment Facilities in the Eastern Cape, South Africa.Food and environmental virology · 2016Article
- Hepatitis E virus is the leading cause of acute viral hepatitis in Lothian, Scotland.New microbes and new infections · 2016Article
- Hepatitis A virus genotype distribution during a decade of universal vaccination of preadolescents.International journal of molecular sciences · 2015Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe hepatitis A virus (HAV) is the most frequent cause of viral hepatitis worldwide and is recognized as one of the most widespread foodborne pathogens. HAV genotypes and subtypes differ in their geographic distribution and the incidence of HAV infection varies considerably among countries, and is particularly high in areas with poor sanitation and hygiene. Phylogenetic analyses are traditionally used in clinical microbiology for tracing the geographic origin of HAV strains. In food microbiology, this approach is complicated by the low contamination levels of food samples. To date, real-time reverse-transcription PCR has been one of the most promising detection methods due to its sensitivity, specificity and ability to deliver quantitative data in food samples, but it does not provide HAV subtyping information.
resultsSix subtype-specific RT-qPCR assays were developed for human HAV. The limit of detection of HAV was 50 genome copies/assay for subtype IIB, 500 genome copies assay for IA, IB, IIA and IIIB and 5000 genome copies/assay for IIIA. The specificity of the assays was evaluated by testing reference isolates and in vitro HAV RNA transcripts. No significant cross reactivity was observed. Subtyping results concordant with sequencing analysis were obtained from 34/35 clinical samples. Co-infection with a minor strain of a different subtype was suggested in 5 cases and a recombinant event in one case.
conclusionsThese RT-qPCR assays may be particularly useful for accurately tracing HAV in low-level contaminated samples such as food matrices but also to allow co-infection identification in human samples.
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