ArticlePloS one2014
Strand Invasion Based Amplification (SIBA®): a novel isothermal DNA amplification technology demonstrating high specificity and sensitivity for a single molecule of target analyte.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Annexing isothermal nucleotide amplification enables probe-guided amplification and direct detection at ambient temperature.Nature communications · 2026Article
- Molecular point-of-care: technological evolution, clinical applications, and future perspectives.Frontiers in bioengineering and biotechnology · 2026Review
- The bacteriophage T4 homologous recombination system: mechanism, applications, conservation, and environmental significance.EcoSal Plus · 2025Review
- Recombinase-Controlled Multiphase Condensates Accelerate Nucleic Acid Amplification and CRISPR-Based Diagnostics.Journal of the American Chemical Society · 2025Article
- Resilient sustainable current and emerging technologies for foodborne pathogen detection.Sustainable food technology · 2025Review
- Integrating Genomic Data with the Development of CRISPR-Based Point-of-Care-Testing for Bacterial Infections.Current clinical microbiology reports · 2024Review
- Manually pressurized droplet digital PCR chip for rapid SARS-CoV-2 diagnostics.Biomicrofluidics · 2024Article
- Engineered helicase replaces thermocycler in DNA amplification while retaining desired PCR characteristics.Nature communications · 2022Article
- Bridging the gap between development of point-of-care nucleic acid testing and patient care for sexually transmitted infections.Lab on a chip · 2022Review
- An improved nucleic acid sequence-based amplification method mediated by T4 gene 32 protein.PloS one · 2022Article
- SARS-CoV-2 detection using reverse transcription strand invasion based amplification and a portable compact size instrument.Scientific reports · 2021Article
- Reopening elite sport during the COVID-19 pandemic: Experiences from a controlled return to elite football in Denmark.Scandinavian journal of medicine & science in sports · 2021Article
- Physical and chemical template-blocking strategies in the exponential amplification reaction of circulating microRNAs.Analytical and bioanalytical chemistry · 2020Article
- Advances in Directly Amplifying Nucleic Acids from Complex Samples.Biosensors · 2019Review
- Detection of human rhinoviruses by reverse transcription strand invasion based amplification method (RT-SIBA).Journal of virological methods · 2019Article
- Molecular Detection of Streptococcus pyogenes by Strand Invasion Based Amplification Assay.Molecular diagnosis & therapy · 2018Article
- Development and evaluation of a rapid nucleic acid amplification method to detect influenza A and B viruses in human respiratory specimens.Diagnostic microbiology and infectious disease · 2018Article
- A new kit to detect Campylobacter species in stool specimens: the Orion GenRead Campylobacter®.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2018Article
- Synergistic and non-specific nucleic acid production by T7 RNA polymerase andSynthetic and systems biotechnology · 2018Article
- Isothermal Amplification of Long, Discrete DNA Fragments Facilitated by Single-Stranded Binding Protein.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Isothermal nucleic acid amplification technologies offer significant advantages over polymerase chain reaction (PCR) in that they do not require thermal cycling or sophisticated laboratory equipment. However, non-target-dependent amplification has limited the sensitivity of isothermal technologies and complex probes are usually required to distinguish between non-specific and target-dependent amplification. Here, we report a novel isothermal nucleic acid amplification technology, Strand Invasion Based Amplification (SIBA). SIBA technology is resistant to non-specific amplification, is able to detect a single molecule of target analyte, and does not require target-specific probes. The technology relies on the recombinase-dependent insertion of an invasion oligonucleotide (IO) into the double-stranded target nucleic acid. The duplex regions peripheral to the IO insertion site dissociate, thereby enabling target-specific primers to bind. A polymerase then extends the primers onto the target nucleic acid leading to exponential amplification of the target. The primers are not substrates for the recombinase and are, therefore unable to extend the target template in the absence of the IO. The inclusion of 2'-O-methyl RNA to the IO ensures that it is not extendible and that it does not take part in the extension of the target template. These characteristics ensure that the technology is resistant to non-specific amplification since primer dimers or mis-priming are unable to exponentially amplify. Consequently, SIBA is highly specific and able to distinguish closely-related species with single molecule sensitivity in the absence of complex probes or sophisticated laboratory equipment. Here, we describe this technology in detail and demonstrate its use for the detection of Salmonella.
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