ArticlePloS one2014
Mechanistic studies of the genetically encoded fluorescent protein voltage probe ArcLight.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Beyond Membrane Potential: Exploiting Signal Complexity in Genetically Encoded Voltage Indicators.Sensors (Basel, Switzerland) · 2026Article
- FLIPs: Genetically encoded molecular biosensors for functional imaging of cell signaling by linear dichroism microscopy.Science advances · 2026Article
- Modulating Chromophore Flexibility in GEVIs through Threonine-Based Molecular Switches Reveals an Influence of Membrane Curvature on Protein Activity.ACS sensors · 2025Article
- Imaging different cell populations in the mouse olfactory bulb using the genetically encoded voltage indicator ArcLight.Neurophotonics · 2024Review
- Cell cycle-linked vacuolar pH dynamics regulate amino acid homeostasis and cell growth.Nature metabolism · 2023Article
- A positively tuned voltage indicator for extended electrical recordings in the brain.Nature methods · 2023Article
- Linearly polarized excitation enhances signals from fluorescent voltage indicators.Biophysical journal · 2021Article
- Monitoring of compound resting membrane potentials of cell cultures with ratiometric genetically encoded voltage indicators.Communications biology · 2021Article
- Mechanism of ArcLight derived GEVIs involves electrostatic interactions that can affect proton wires.Biophysical journal · 2021Article
- Quantitative linear dichroism imaging of molecular processes in living cells made simple by open software tools.Communications biology · 2021Article
- Visualizing Oscillations in Brain Slices With Genetically Encoded Voltage Indicators.Frontiers in neuroanatomy · 2021Article
- Directionality of light absorption and emission in representative fluorescent proteins.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Biophysical Parameters of GEVIs: Considerations for Imaging Voltage.Biophysical journal · 2020Review
- Genetic voltage indicators.BMC biology · 2019Review
- Optical consequences of a genetically-encoded voltage indicator with a pH sensitive fluorescent protein.Neuroscience research · 2019Review
- Evidence That Ion-Based Signaling Initiating at the Cell Surface Can Potentially Influence Chromatin Dynamics and Chromatin-Bound Proteins in the Nucleus.Frontiers in plant science · 2019Article
- Optimizing Strategies for Developing Genetically Encoded Voltage Indicators.Frontiers in cellular neuroscience · 2019Review
- Using Genetically Encoded Voltage Indicators (GEVIs) to Study the Input-Output Transformation of the Mammalian Olfactory Bulb.Frontiers in cellular neuroscience · 2019Article
- A dimeric fluorescent protein yields a bright, red-shifted GEVI capable of population signals in brain slice.Scientific reports · 2018Article
- Genetically encoded fluorescent voltage indicators: are we there yet?Current opinion in neurobiology · 2018Review
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Authors and funding
8 authors.
Funding
Abstract
ArcLight, a genetically encoded fluorescent protein voltage probe with a large ΔF/ΔV, is a fusion between the voltage sensing domain of the Ciona instestinalis voltage sensitive phosphatase and super ecliptic pHluorin carrying a single mutation (A227D in the fluorescent protein). Without this mutation the probe produces only a very small change in fluorescence in response to voltage deflections (∼ 1%). The large signal afforded by this mutation allows optical detection of action potentials and sub-threshold electrical events in single-trials in vitro and in vivo. However, it is unclear how this single mutation produces a probe with such a large modulation of its fluorescence output with changes in membrane potential. In this study, we identified which residues in super ecliptic pHluorin (vs eGFP) are critical for the ArcLight response, as a similarly constructed probe based on eGFP also exhibits large response amplitude if it carries these critical residues. We found that D147 is responsible for determining the pH sensitivity of the fluorescent protein used in these probes but by itself does not result in a voltage probe with a large signal. We also provide evidence that the voltage dependent signal of ArcLight is not simply sensing environmental pH changes. A two-photon polarization microscopy study showed that ArcLight's response to changes in membrane potential includes a reorientation of the super ecliptic pHluorin. We also explored different changes including modification of linker length, deletion of non-essential amino acids in the super ecliptic pHluorin, adding a farnesylation site, using tandem fluorescent proteins and other pH sensitive fluorescent proteins.
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Registered trials
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