ArticlePhysical review applied2014
Three-Dimensional Holographic Refractive-Index Measurement of Continuously Flowing Cells in a Microfluidic Channel.
Article in Physical review applied, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
38 citing papers in PubMed, 111 citations in OpenAlex.
- Three-dimensional imaging flow cytometry: current trends and future perspectives.Lab on a chip · 2026Review
- Speckle-based measurement of the fractional azimuthal index of orbital angular momentum beams for refractive index sensing.Nature communications · 2026Article
- Review
- Towards ultrafast quantitative phase imaging via differentiable microscopy [Invited].Biomedical optics express · 2024Article
- Recent Advances and Current Trends in Transmission Tomographic Diffraction Microscopy.Sensors (Basel, Switzerland) · 2024Review
- Utilizing 3D Models to Unravel the Dynamics of Myeloma Plasma Cells' Escape from the Bone Marrow Microenvironment.Cancers · 2024Review
- Label-free liquid biopsy through the identification of tumor cells by machine learning-powered tomographic phase imaging flow cytometry.Scientific reports · 2023Article
- Bond-selective intensity diffraction tomography.Nature communications · 2022Article
- Stain-free identification of cell nuclei using tomographic phase microscopy in flow cytometry.Nature photonics · 2022Article
- A high-throughput technique to map cell images to cell positions using a 3D imaging flow cytometer.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Snapshot three-dimensional absorption imaging of microscopic specimens.Physical review applied · 2021Article
- Holotomography: Refractive Index as an Intrinsic Imaging Contrast for 3-D Label-Free Live Cell Imaging.Advances in experimental medicine and biology · 2021Article
- Recent advances in point spread function engineering and related computational microscopy approaches: from one viewpoint.Biophysical reviews · 2020Review
- Lab-on-a-Chip Technologies for the Single Cell Level: Separation, Analysis, and Diagnostics.Micromachines · 2020Review
- Label-free imaging flow cytometer for analyzing large cell populations by line-field quantitative phase microscopy with digital refocusing.Biomedical optics express · 2020Article
- Reconstruction of bovine spermatozoa substances distribution and morphological differences between Holstein and Korean native cattle using three-dimensional refractive index tomography.Scientific reports · 2019Article
- Wavelet compression of off-axis digital holograms using real/imaginary and amplitude/phase parts.Scientific reports · 2019Article
- Low-coherent optical diffraction tomography by angle-scanning illumination.Journal of biophotonics · 2019Article
- Direct Three-Dimensional Measurement of Refractive Index via Dual Photon-Phonon Scattering.Physical review letters · 2019Article
- Invited Article: Digital refocusing in quantitative phase imaging for flowing red blood cells.APL photonics · 2018Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
Refractive index of biological specimens is a source of intrinsic contrast that can be explored without any concerns of photobleaching or harmful effects caused by extra contrast agents. In addition, RI contains rich information related to the metabolism of cells at the cellular and subcellular levels. Here, we report a no-moving parts approach that provides three-dimensional refractive index maps of biological samples continuously flowing in a microfluidic channel. Specifically, we use line illumination and off-axis digital holography to record the angular spectra of light scattered from flowing samples at high speed. Applying the scalar diffraction theory, we obtain accurate RI maps of the samples from the measured spectra. Using this method, we demonstrate label-free 3-D imaging of live RKO human colon cancer cells and RPMI8226 multiple myeloma cells, and obtain the volume, dry mass and density of these cells from the measured 3-D refractive index maps. Our results show that the reported method, alone or in combination with the existing flow cytometry techniques, promises as a quantitative tool for stain-free characterization of large number of cells.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.