ArticleMethods and applications in fluorescence2014
Orbital Single Particle Tracking on a commercial confocal microscope using piezoelectric stage feedback.
Article in Methods and applications in fluorescence, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- 3D Single-Virus Tracking: Advances in Methodology and Labeling Strategies Towards Probing the Virus-Epithelium Interaction.Viruses · 2026Review
- Parameter optimization for MINFLUX microscopy enabled single particle tracking.Communications biology · 2025Article
- Timing is everything: transcription bursting in development.Genes & development · 2025Review
- Recent Advances in Single-Molecule Tracking and Imaging Techniques.Annual review of analytical chemistry (Palo Alto, Calif.) · 2023Review
- Impact ofInternational journal of molecular sciences · 2022Review
- Computational Proposal for Tracking Multiple Molecules in a Multifocus Confocal Setup.ACS photonics · 2022Article
- Real-Time Feedback-Driven Single-Particle Tracking: A Survey and Perspective.Small (Weinheim an der Bergstrasse, Germany) · 2022Review
- Advances in fluorescence microscopy techniques to study kidney function.Nature reviews. Nephrology · 2021Review
- Feedforward Control for Single Particle Tracking Synthetic Motion.IFAC-PapersOnLine · 2020Article
- Real-Time 3D Single Particle Tracking: Towards Active Feedback Single Molecule Spectroscopy in Live Cells.Molecules (Basel, Switzerland) · 2019Review
- Measuring Mobility in Chromatin by Intensity-Sorted FCS.Biophysical journal · 2019Article
- Fluctuation Spectroscopy Analysis of Glucose Capped Gold Nanoparticles.Langmuir : the ACS journal of surfaces and colloids · 2016Article
- Phasor Analysis of Local ICS Detects Heterogeneity in Size and Number of Intracellular Vesicles.Biophysical journal · 2016Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Single Particle Tracking (SPT) is a technique used to locate fluorescent particles with nanometer precision. In the orbital tracking method the position of a particle is obtained analyzing the distribution of intensity along a circular orbit scanned around the particle. In combination with an active feedback this method allows tracking of particles in 2D and 3D with millisecond temporal resolution. Here we describe a SPT setup based on a feedback approach implemented with minimal modification of a commercially available confocal laser scanning microscope, the Zeiss LSM 510, in combination with an external piezoelectric stage scanner. The commercial microscope offers the advantage of a user-friendly software interface and pre-calibrated hardware components. The use of an external piezo-scanner allows the addition of feedback into the system but also represents a limitation in terms of its mechanical response. We describe in detail this implementation of the orbital tracking method and discuss advantages and limitations. As an example of application to live cell experiments we perform the 3D tracking of acidic vesicles in live polarized epithelial cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.