ReviewMolecular bioSystems2015
Enzymatic strategies and biocatalysts for amide bond formation: tricks of the trade outside of the ribosome.
Review in Molecular bioSystems, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Alternative enzymatic pathways to penicillin antibiotics.Nature communications · 2026Article
- Unlocking the Silent Proteome: Chemoselective Asn/Gln Activation for Multidimensional Protein Diversification.Journal of the American Chemical Society · 2026Article
- Article
- Synthetic Biology in Natural Product Biosynthesis.Chemical reviews · 2025Review
- Selective peptide bond formation via side chain reactivity and self-assembly of abiotic phosphates.Nature communications · 2025Article
- Defect-Engineered Metal-Organic Frameworks as Bioinspired Heterogeneous Catalysts for Amide Bond Formation.Journal of the American Chemical Society · 2024Article
- Process Mass Intensity (PMI): A Holistic Analysis of Current Peptide Manufacturing Processes Informs Sustainability in Peptide Synthesis.The Journal of organic chemistry · 2024Review
- Broad Spectrum Enantioselective Amide Bond Synthetase fromACS catalysis · 2024Article
- Enzymatic Fluoromethylation as a Tool for ATP-Independent Ligation.Angewandte Chemie (International ed. in English) · 2024Article
- Enzymatic Fluoromethylation as a Tool for ATP-Independent Ligation.Angewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2024Article
- Selective amide bond formation in redox-active coacervate protocells.Nature communications · 2023Article
- Fast Solution-Phase and Liquid-Phase Peptide Syntheses (SolPSS and LPPS) Mediated by Biomimetic Cyclic Propylphosphonic Anhydride (T3PMolecules (Basel, Switzerland) · 2023Article
- Structural basis of the amidase ClbL central to the biosynthesis of the genotoxin colibactin.Acta crystallographica. Section D, Structural biology · 2023Article
- N-Amidation of Nitrogen-Containing Heterocyclic Compounds: Can We Apply Enzymatic Tools?Bioengineering (Basel, Switzerland) · 2023Review
- An unprecedented palladium-arsenic catalytic cycle for nitriles hydration.Frontiers in chemistry · 2023Article
- One-Step Biocatalytic Synthesis of Sustainable Surfactants by Selective Amide Bond Formation.Angewandte Chemie (International ed. in English) · 2022Article
- Review
- The Transformative Power of Biocatalysis in Convergent Synthesis.Journal of the American Chemical Society · 2022Review
- Merging enzymes with chemocatalysis for amide bond synthesis.Nature communications · 2022Article
- A [3Fe-4S] cluster and tRNA-dependent aminoacyltransferase BlsK in the biosynthesis of Blasticidin S.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Amide bond-containing (ABC) biomolecules are some of the most intriguing and functionally significant natural products with unmatched utility in medicine, agriculture and biotechnology. The enzymatic formation of an amide bond is therefore a particularly interesting platform for engineering the synthesis of structurally diverse natural and unnatural ABC molecules for applications in drug discovery and molecular design. As such, efforts to unravel the mechanisms involved in carboxylate activation and substrate selection has led to the characterization of a number of structurally and functionally distinct protein families involved in amide bond synthesis. Unlike ribosomal synthesis and thio-templated synthesis using nonribosomal peptide synthetases, which couple the hydrolysis of phosphoanhydride bond(s) of ATP and proceed via an acyl-adenylate intermediate, here we discuss two mechanistically alternative strategies: ATP-dependent enzymes that generate acylphosphate intermediates and ATP-independent transacylation strategies. Several examples highlighting the function and synthetic utility of these amide bond-forming strategies are provided.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.