ArticleStem cell reports2014
Manipulation of KLF4 expression generates iPSCs paused at successive stages of reprogramming.
Article in Stem cell reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 56 citations in OpenAlex.
- Ubiquitin-Derived Fragment as a Peptide Linker for the Efficient Cleavage of a Target Protein from a Degron.ACS chemical biology · 2024Article
- An engineered ligand-responsive Csy4 endoribonuclease controls transgene expression from Sendai virus vectors.Journal of biological engineering · 2024Article
- Synthetic genetic circuits to uncover the OCT4 trajectories of successful reprogramming of human fibroblasts.Science advances · 2023Article
- Predicting reprogramming-related gene expression from cell morphology in human induced pluripotent stem cells.Molecular biology of the cell · 2023Article
- Locus-Specific Isolation of theInternational journal of molecular sciences · 2022Article
- Comprehensive comparison of gene expression diversity among a variety of human stem cells.NAR genomics and bioinformatics · 2022Article
- miRNA-mediated control of exogenousMolecular therapy. Methods & clinical development · 2022Article
- Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency.iScience · 2022Article
- Early reactivation of clustered genes on the inactive X chromosome during somatic cell reprogramming.Stem cell reports · 2022Article
- Liquid condensation of reprogramming factor KLF4 with DNA provides a mechanism for chromatin organization.Nature communications · 2021Article
- Nanofountain Probe Electroporation Enables Versatile Single-Cell Intracellular Delivery and Investigation of Postpulse Electropore Dynamics.Small (Weinheim an der Bergstrasse, Germany) · 2020Article
- Impact of theCancers · 2020Article
- Robust and highly efficient hiPSC generation from patient non-mobilized peripheral blood-derived CD34Stem cell research & therapy · 2019Article
- Live-cell imaging of subcellular structures for quantitative evaluation of pluripotent stem cells.Scientific reports · 2019Article
- Exploring induced pluripotency in human fibroblasts via construction, validation, and application of a gene regulatory network.PloS one · 2019Article
- Universal intracellular biomolecule delivery with precise dosage control.Science advances · 2018Article
- Live cell imaging of X chromosome reactivation during somatic cell reprogramming.Biochemistry and biophysics reports · 2018Article
- Time-course transcriptome analysis of human cellular reprogramming from multiple cell types reveals the drastic change occurs between the mid phase and the late phase.BMC genomics · 2018Article
- Reprogramming of mouse fibroblasts into neural lineage cells using biomaterials.BioImpacts : BI · 2018Article
- Sensitive and long-term monitoring of intracellular microRNAs using a non-integrating cytoplasmic RNA vector.Scientific reports · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The detailed mechanism of reprogramming somatic cells into induced pluripotent stem cells (iPSCs) remains largely unknown. Partially reprogrammed iPSCs are informative and useful for understanding the mechanism of reprogramming but remain technically difficult to generate in a predictable and reproducible manner. Using replication-defective and persistent Sendai virus (SeVdp) vectors, we analyzed the effect of decreasing the expression levels of OCT4, SOX2, KLF4, and c-MYC and found that low KLF4 expression reproducibly gives rise to a homogeneous population of partially reprogrammed iPSCs. Upregulation of KLF4 allows these cells to resume reprogramming, indicating that they are paused iPSCs that remain on the path toward pluripotency. Paused iPSCs with different KLF4 expression levels remain at distinct intermediate stages of reprogramming. This SeVdp-based stage-specific reprogramming system (3S reprogramming system) is applicable for both mouse and human somatic cells and will facilitate the mechanistic analysis of reprogramming.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.