ArticleScientific reports2014
Cloning and characterization of wnt4a gene and evidence for positive selection in half-smooth tongue sole (Cynoglossus semilaevis).
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 36 citations in OpenAlex.
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- Comparative analysis of plastid genomes within the Campanulaceae and phylogenetic implications.PloS one · 2020Article
- Comparative transcriptome profiling of immune response against Vibrio harveyi infection in Chinese tongue sole.Scientific data · 2019Article
- The Model of the Conserved Epigenetic Regulation of Sex.Frontiers in genetics · 2019Article
- The autosomal Gsdf gene plays a role in male gonad development in Chinese tongue sole (Cynoglossus semilaevis).Scientific reports · 2018Article
- MiR-202 controls female fecundity by regulating medaka oogenesis.PLoS genetics · 2018Article
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- Identification and analysis of the β-catenin1 gene in half-smooth tongue sole (Cynoglossus semilaevis).PloS one · 2017Article
- Characterization and tissue distribution of Lhx9 and Lhx9α in Chinese giant salamander Andrias davidianus.Journal of genetics · 2016Article
- Molecular cloning and sexually dimorphic expression of wnt4 in olive flounder (Paralichthys olivaceus).Fish physiology and biochemistry · 2016Article
- Two Figla homologues have disparate functions during sex differentiation in half-smooth tongue sole (Cynoglossus semilaevis).Scientific reports · 2016Article
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5 authors at 2 institutions in 1 country.
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Abstract
Wnt4 gene plays a role in developmental processes in mammals. However, little is known regarding its function in teleosts. We cloned and characterized the full-length half-smooth tongue sole (Cynoglossus semilaevis) wnt4a gene (CS-wnt4a). CS-wnt4a cDNA was 1746 bp in length encoding 353aa. CS-wnt4a expression level was highest in the testis, and gradually increased in the developing gonads until 1 year of age. In situ hybridization revealed that CS-wnt4a expression level was highest in stage II oocytes and sperm in the adult ovary and testis, respectively. CS-wnt4a expression level was significantly up-regulated in the gonads after exposure to high temperature. The level of methylation of the CS-wnt4a first exon was negatively correlated with the expression of CS-wnt4a. The branch-site model suggested that vertebrate wnt4a differed significantly from that of wnt4b, and that the selective pressures differed between ancestral aquatic and terrestrial organisms. Two positively selected sites were found in the ancestral lineages of teleost fish, but none in the ancestral lineages of mammals. One positively selected site was located on the α-helices of the 3D structure, the other on the random coil. Our results are of value for further study of the function of wnt4 and the mechanism of selection.
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