ArticleBiophysical journal2014
Polarized THG microscopy identifies compositionally different lipid droplets in mammalian cells.
Article in Biophysical journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 33 citations in OpenAlex.
- Exploring liquid-liquid phase separation in vitro and in vivo using multimodal nonlinear optical imaging.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025Article
- Nuclear envelope-associated lipid droplets are enriched in cholesteryl esters and increase during inflammatory signaling.The EMBO journal · 2025Article
- Third harmonic imaging contrast from tubular structures in the presence of index discontinuity.Scientific reports · 2023Article
- Cancer metabolic features allow discrimination of tumor from white blood cells by label-free multimodal optical imaging.Frontiers in bioengineering and biotechnology · 2023Article
- Label-free third harmonic generation imaging and quantification of lipid droplets in live filamentous fungi.Scientific reports · 2022Article
- Recent Advancements in Optical Harmonic Generation Microscopy: Applications and Perspectives.BME frontiers · 2021Review
- Universal phase behaviors of intracellular lipid droplets.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Foam Cells: One Size Doesn't Fit All.Trends in immunology · 2019Review
- Dynamic Remodeling of the Host Cell Membrane by Virulent Mycobacterial Sulfoglycolipid-1.Scientific reports · 2019Article
- Article
- Storage lipid studies in tuberculosis reveal that foam cell biogenesis is disease-specific.PLoS pathogens · 2018Article
- Label-free imaging of atherosclerotic plaques using third-harmonic generation microscopy.Biomedical optics express · 2018Article
- Lipid droplet functions beyond energy storage.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2017Review
- Role for formin-like 1-dependent acto-myosin assembly in lipid droplet dynamics and lipid storage.Nature communications · 2017Article
- miR-29a Promotes Lipid Droplet and Triglyceride Formation in HCV Infection by Inducing Expression of SREBP-1c and CAV1.Journal of clinical and translational hepatology · 2016Article
- D38-cholesterol as a Raman active probe for imaging intracellular cholesterol storage.Journal of biomedical optics · 2016Article
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Authors and funding
7 authors at 4 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Cells store excess lipids as two major compounds, triacylglycerols (TAGs) and cholesteryl esters (CEs), inside lipid droplets (LDs). The degree of lipid ordering is considered to play a major role in the mobility and enzymatic processing of lipids in LDs. Here, we provide evidence that polarized third-harmonic generation (THG) microscopy distinguishes between native TAG- and CE-enriched LDs in cells due to the different ordering of the two lipid species. We first demonstrate that the responses from synthetic TAG- and CE-enriched LDs using THG microscopy with linear and circular polarizations differ according to their different intrinsic ordering. We then employ simulations to dissect how polarization effects influence the THG from an isotropic LD. Finally, we induce TAG- and CE-enriched LDs in murine macrophages and demonstrate that polarized THG responses increase in a nonlinear fashion with increasing CE/TAG ratio. This suggests that with an increasing CE content, there is a rather sharp transition toward increased LD ordering. Our results demonstrate that polarized THG microscopy enables label-free quantitative analysis of LD ordering and discriminates between compositionally different LDs in intact mammalian cells.
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