ArticleBiophysical journal2014
Quantitatively imaging chromosomes by correlated cryo-fluorescence and soft x-ray tomographies.
Article in Biophysical journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 81 citations in OpenAlex.
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- Endoplasmic reticulum stress triggers unfolded protein response as an antiviral strategy of teleost erythrocytes.Frontiers in immunology · 2024Article
- On-Line Fluorescence Microscopy for Identification and Imaging of Apoptotic Cell with Synchrotron-Based Soft X-ray Tomography.Micromachines · 2023Article
- Correlative Cryo-imaging Using Soft X-Ray Tomography for the Study of Virus Biology in Cells and Tissues.Sub-cellular biochemistry · 2023Article
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- Combined optical fluorescence microscopy and X-ray tomography reveals substructures in cell nuclei in 3D.Biomedical optics express · 2022Article
- Soft X-ray tomography to map and quantify organelle interactions at the mesoscale.Structure (London, England : 1993) · 2022Article
- A protocol for full-rotation soft X-ray tomography of single cells.STAR protocols · 2022Article
- Auto-segmentation and time-dependent systematic analysis of mesoscale cellular structure in β-cells during insulin secretion.PloS one · 2022Article
- Using soft X-ray tomography for rapid whole-cell quantitative imaging of SARS-CoV-2-infected cells.Cell reports methods · 2021Article
- Sample preparation strategies for efficient correlation of 3D SIM and soft X-ray tomography data at cryogenic temperatures.Nature protocols · 2021Article
- X-ray Ptychography Imaging of Human Chromosomes After Low-dose Irradiation.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2021Article
- Three-dimensional imaging of mitochondrial cristae complexity using cryo-soft X-ray tomography.Scientific reports · 2020Article
- Assaying three-dimensional cellular architecture using X-ray tomographic and correlated imaging approaches.The Journal of biological chemistry · 2020Review
- Computational approaches for inferring 3D conformations of chromatin from chromosome conformation capture data.Methods (San Diego, Calif.) · 2020Review
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- Nanoscopic X-ray tomography for correlative microscopy of a small meiofaunal sea-cucumber.Scientific reports · 2020Article
- Soft X-ray tomography: virtual sculptures from cell cultures.Current opinion in structural biology · 2019Review
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7 authors at 2 institutions in 1 country.
Funding
Abstract
Soft x-ray tomography (SXT) is increasingly being recognized as a valuable method for visualizing and quantifying the ultrastructure of cryopreserved cells. Here, we describe the combination of SXT with cryogenic confocal fluorescence tomography (CFT). This correlative approach allows the incorporation of molecular localization data, with isotropic precision, into high-resolution three-dimensional (3-D) SXT reconstructions of the cell. CFT data are acquired first using a cryogenically adapted confocal light microscope in which the specimen is coupled to a high numerical aperture objective lens by an immersion fluid. The specimen is then cryo-transferred to a soft x-ray microscope (SXM) for SXT data acquisition. Fiducial markers visible in both types of data act as common landmarks, enabling accurate coalignment of the two complementary tomographic reconstructions. We used this method to identify the inactive X chromosome (Xi) in female v-abl transformed thymic lymphoma cells by localizing enhanced green fluorescent protein-labeled macroH2A with CFT. The molecular localization data were used to guide segmentation of Xi in the SXT reconstructions, allowing characterization of the Xi topological arrangement in near-native state cells. Xi was seen to adopt a number of different topologies with no particular arrangement being dominant.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.