ArticleBiophysical journal2014
Origins of Escherichia coli growth rate and cell shape changes at high external osmolality.
Article in Biophysical journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 27 citations in OpenAlex.
- Emergent collective alignment gives competitive advantage to longer cells during range expansion.Nature communications · 2025Article
- Extracellular peptide production in Escherichia coli by inducible downregulation of lipoprotein Lpp via MicL sRNA.Applied microbiology and biotechnology · 2025Article
- Unraveling the impact of pH, sodium concentration, and medium osmolality on Vibrio natriegens in batch processes.BMC biotechnology · 2024Article
- Delaying production with prokaryotic inducible expression systems.Microbial cell factories · 2024Review
- Recombinant Peptide Production SoftensInternational journal of molecular sciences · 2023Article
- Steady-state running rate sets the speed and accuracy of accumulation of swimming bacteria.Biophysical journal · 2022Article
- Lpp positions peptidoglycan at the AcrA-TolC interface in the AcrAB-TolC multidrug efflux pump.Biophysical journal · 2021Article
- Robust surface-to-mass coupling and turgor-dependent cell width determine bacterial dry-mass density.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Understanding Beta-Lactam-Induced Lysis at the Single-Cell Level.Frontiers in microbiology · 2021Article
- Intracellular ion concentrations and cation-dependent remodelling of bacterial MreB assemblies.Scientific reports · 2020Article
- Lipoprotein Lpp regulates the mechanical properties of the E. coli cell envelope.Nature communications · 2020Article
- L form bacteria growth in low-osmolality medium.Microbiology (Reading, England) · 2019Article
- Decreased Effective Macromolecular Crowding in Escherichia coli Adapted to Hyperosmotic Stress.Journal of bacteriology · 2019Article
- Electrooptical Determination of Polarizability for On-Line Viability and Vitality Quantification ofFrontiers in bioengineering and biotechnology · 2018Article
- Fluorescent D-amino-acids reveal bi-cellular cell wall modifications important for Bdellovibrio bacteriovorus predation.Nature microbiology · 2017Article
- Osmotaxis inProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Cell-Biological Studies of Osmotic Shock Response in Streptomyces spp.Journal of bacteriology · 2017Article
- Dynamics of Escherichia coli's passive response to a sudden decrease in external osmolarity.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Constraints, Trade-offs and the Currency of Fitness.Journal of molecular evolution · 2016Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 2 countries.
Funding
Abstract
In Escherichia coli, a sudden increase in external concentration causes a pressure drop across the cell envelope, followed by an active recovery. After recovery, and if the external osmolality remains high, cells have been shown to grow more slowly, smaller, and at reduced turgor pressure. Despite the fact that the active recovery is a key stress response, the nature of these changes and how they relate to each other is not understood. Here, we use fluorescence imaging of single cells during hyperosmotic shocks, combined with custom made microfluidic devices, to show that cells fully recover their volume to the initial, preshock value and continue to grow at a slower rate immediately after the recovery. We show that the cell envelope material properties do not change after hyperosmotic shock, and that cell shape recovers along with cell volume. Taken together, these observations indicate that the turgor pressure recovers to its initial value so that reduced turgor is not responsible for the reduced growth rate observed immediately after recovery. To determine the point at which the reduction in cell size and turgor pressure occurs after shock, we measured the volume of E. coli cells at different stages of growth in bulk cultures. We show that cell volume reaches the same maximal level irrespective of the osmolality of the media. Based on these measurements, we propose that turgor pressure is used as a feedback variable for osmoregulatory pumps instead of being directly responsible for the reduction in growth rates. Reestablishment of turgor to its initial value might ensure correct attachment of the inner membrane and cell wall needed for cell wall biosynthesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.