ArticleeLife2014
Effective use of a horizontally-transferred pathway for dichloromethane catabolism requires post-transfer refinement.
Article in eLife, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed.
- EngineeredmSphere · 2025Article
- Considering Metabolic Context in Enzyme Evolution and Design.Biochemistry · 2025Review
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- The genetic landscape of a metabolic interaction.Nature communications · 2024Article
- Engineering Proteins Using Statistical Models of Coevolutionary Sequence Information.Cold Spring Harbor perspectives in biology · 2024Review
- Genome-Wide Transcription Start Sites Mapping inMicroorganisms · 2022Article
- The role of mobile genetic elements in organic micropollutant degradation during biological wastewater treatment.Water research X · 2020Review
- Dichloromethane Degradation Pathway from UnsequencedMicroorganisms · 2020Article
- Evolutionary stalling and a limit on the power of natural selection to improve a cellular module.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Horizontal transfer of a pathway for coumarate catabolism unexpectedly inhibits purine nucleotide biosynthesis.Molecular microbiology · 2019Article
- Continuous Culture Adaptation ofFrontiers in microbiology · 2019Article
- Salt Adaptation and Evolutionary Implication of a Nah-related PAHs Dioxygenase cloned from a Halophilic Phenanthrene Degrading Consortium.Scientific reports · 2017Article
- Biodegradation of Volatile Organic Compounds and Their Effects on Biodegradability under Co-Existing Conditions.Microbes and environments · 2017Review
- Construction and Optimization of a Heterologous Pathway for Protocatechuate Catabolism in Escherichia coli Enables Bioconversion of Model Aromatic Compounds.Applied and environmental microbiology · 2017Article
- Genomic and Transcriptomic Analysis of Growth-Supporting Dehalogenation of Chlorinated Methanes inFrontiers in microbiology · 2017Article
- Accepting Foreign Genes.Journal of molecular evolution · 2016Review
- Transfer of a Catabolic Pathway for Chloromethane in Methylobacterium Strains Highlights Different Limitations for Growth with Chloromethane or with Dichloromethane.Frontiers in microbiology · 2016Article
- Exploring the Ancestral Mechanisms of Regulation of Horizontally Acquired Nitrogenases.Journal of molecular evolution · 2015Article
- Parallel and Divergent Evolutionary Solutions for the Optimization of an Engineered Central Metabolism in Methylobacterium extorquens AM1.Microorganisms · 2015Article
- Article
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Authors and funding
5 authors.
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Abstract
When microbes acquire new abilities through horizontal gene transfer, the genes and pathways must function under conditions with which they did not coevolve. If newly-acquired genes burden the host, their utility will depend on further evolutionary refinement of the recombinant strain. We used laboratory evolution to recapitulate this process of transfer and refinement, demonstrating that effective use of an introduced dichloromethane degradation pathway required one of several mutations to the bacterial host that are predicted to increase chloride efflux. We then used this knowledge to identify parallel, beneficial mutations that independently evolved in two natural dichloromethane-degrading strains. Finally, we constructed a synthetic mobile genetic element carrying both the degradation pathway and a chloride exporter, which preempted the adaptive process and directly enabled effective dichloromethane degradation across diverse Methylobacterium environmental isolates. Our results demonstrate the importance of post-transfer refinement in horizontal gene transfer, with potential applications in bioremediation and synthetic biology.
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