ArticleScientific reports2014
Low piconewton towing of CNS axons against diffusing and surface-bound repellents requires the inhibition of motor protein-associated pathways.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it.
- The effect of nanomaterials on embryonic stem cell neural differentiation: a systematic review.European journal of medical research · 2023Pooled it
- Advanced materials and devices strategies of mechanical stimulation for tissue regeneration.Regenerative biomaterials · 2026Review
- Manipulation of New Fluorescent Magnetic Nanoparticles with an Electromagnetic Needle, Allowed Determining the Viscosity of the Cytoplasm of M-HeLa Cells.Pharmaceuticals (Basel, Switzerland) · 2023Article
- Magnetically-actuated microposts stimulate axon growth.Biophysical journal · 2022Article
- miR-30b Promotes spinal cord sensory function recovery via the Sema3A/NRP-1/PlexinA1/RhoA/ROCK Pathway.Journal of cellular and molecular medicine · 2020Article
- Manipulation of Axonal Outgrowth via Exogenous Low Forces.International journal of molecular sciences · 2020Review
- Extremely Low Forces Induce Extreme Axon Growth.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- The Myosin Family of Mechanoenzymes: From Mechanisms to Therapeutic Approaches.Annual review of biochemistry · 2020Review
- Growth and elongation of axons through mechanical tension mediated by fluorescent-magnetic bifunctional FeJournal of nanobiotechnology · 2020Article
- Pyk2 overexpression in postsynaptic neurons blocks amyloid βBrain communications · 2020Article
- Mechanotransduction in neuronal cell development and functioning.Biophysical reviews · 2019Review
- An Integrated Cytoskeletal Model of Neurite Outgrowth.Frontiers in cellular neuroscience · 2018Review
- Kinesin-5 Blocker Monastrol Protects Against Bortezomib-Induced Peripheral Neurotoxicity.Neurotoxicity research · 2017Article
- Modulating motility of intracellular vesicles in cortical neurons with nanomagnetic forces on-chip.Lab on a chip · 2017Article
- Bio-Nano-Magnetic Materials for Localized Mechanochemical Stimulation of Cell Growth and Death.Advanced materials (Deerfield Beach, Fla.) · 2016Article
- Neuronal Cell Bodies Remotely Regulate Axonal Growth Response to Localized Netrin-1 Treatment via Second Messenger and DCC Dynamics.Frontiers in cellular neuroscience · 2016Article
- Non-muscle myosin II in disease: mechanisms and therapeutic opportunities.Disease models & mechanisms · 2015Review
- Inhibition of kinesin-5 improves regeneration of injured axons by a novel microtubule-based mechanism.Neural regeneration research · 2015Review
- Traction force and tension fluctuations in growing axons.Frontiers in cellular neuroscience · 2015Article
- Microtechnologies for studying the role of mechanics in axon growth and guidance.Frontiers in cellular neuroscience · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Growth cones, dynamic structures at axon tips, integrate chemical and physical stimuli and translate them into coordinated axon behaviour, e.g., elongation or turning. External force application to growth cones directs and enhances axon elongation in vitro; however, direct mechanical stimulation is rarely combined with chemotactic stimulation. We describe a microfluidic device that exposes isolated cortical axons to gradients of diffusing and substrate-bound molecules, and permits the simultaneous application of piconewton (pN) forces to multiple individual growth cones via magnetic tweezers. Axons treated with Y-27632, a RhoA kinase inhibitor, were successfully towed against Semaphorin 3A gradients, which repel untreated axons, with less than 12 pN acting on a small number of neural cell adhesion molecules. Treatment with Y-27632 or monastrol, a kinesin-5 inhibitor, promoted axon towing on substrates coated with chondroitin sulfate proteoglycans, potent axon repellents. Thus, modulating key molecular pathways that regulate contractile stress generation in axons counteracts the effects of repellent molecules and promotes tension-induced growth. The demonstration of parallel towing of axons towards inhibitory environments with minute forces suggests that mechanochemical stimulation may be a promising therapeutic approach for the repair of the damaged central nervous system, where regenerating axons face repellent factors over-expressed in the glial scar.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.