ArticleNucleic acids research2014
Spatial organization of transcription machinery and its segregation from the replisome in fast-growing bacterial cells.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 25 citations in OpenAlex.
- Induced DNA double strand breaks by genotoxic drugs occur at active transcription H3K36 tri-methylation sites.Communications biology · 2026Article
- Omega stabilizes RNA polymerase condensates and contributes to cellular fitness during acid stress.bioRxiv : the preprint server for biology · 2026Article
- LsrL modulates Lsr2-induced chromatin structure to tune biosynthetic gene cluster regulation inbioRxiv : the preprint server for biology · 2025Article
- RNA polymerase redistribution supports growth in E. coli strains with a minimal number of rRNA operons.Nucleic acids research · 2023Article
- Clusters of bacterial RNA polymerase are biomolecular condensates that assemble through liquid-liquid phase separation.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Density of σ70 promoter-like sites in the intergenic regions dictates the redistribution of RNA polymerase during osmotic stress in Escherichia coli.Nucleic acids research · 2019Article
- Successive Paradigm Shifts in the Bacterial Cell Cycle and Related Subjects.Life (Basel, Switzerland) · 2019Article
- High-Copy-Number Plasmid Segregation-Single-Molecule Dynamics in Single Cells.Biophysical journal · 2019Article
- Extrachromosomal Nucleolus-Like Compartmentalization by a Plasmid-Borne Ribosomal RNA Operon and Its Role in Nucleoid Compaction.Frontiers in microbiology · 2018Article
- Nucleolus-like compartmentalization of the transcription machinery in fast-growing bacterial cells.Critical reviews in biochemistry and molecular biology · 2017Review
- Species-specific supercoil dynamics of the bacterial nucleoid.Biophysical reviews · 2016Article
- Optical and force nanoscopy in microbiology.Nature microbiology · 2016Review
- Perturbed states of the bacterial chromosome: a thymineless death case study.Frontiers in microbiology · 2015Review
- Regulation of Transcript Elongation.Annual review of microbiology · 2015Review
- The dynamic nature and territory of transcriptional machinery in the bacterial chromosome.Frontiers in microbiology · 2015Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
In a fast-growing Escherichia coli cell, most RNA polymerase (RNAP) is allocated to rRNA synthesis forming transcription foci at clusters of rrn operons or bacterial nucleolus, and each of the several nascent nucleoids contains multiple pairs of replication forks. The composition of transcription foci has not been determined. In addition, how the transcription machinery is three-dimensionally organized to promote cell growth in concord with replication machinery in the nucleoid remains essentially unknown. Here, we determine the spatial and functional landscapes of transcription and replication machineries in fast-growing E. coli cells using super-resolution-structured illumination microscopy. Co-images of RNAP and DNA reveal spatial compartmentation and duplication of the transcription foci at the surface of the bacterial chromosome, encompassing multiple nascent nucleoids. Transcription foci cluster with NusA and NusB, which are the rrn anti-termination system and are associated with nascent rRNAs. However, transcription foci tend to separate from SeqA and SSB foci, which track DNA replication forks and/or the replisomes, demonstrating that transcription machinery and replisome are mostly located in different chromosomal territories to maintain harmony between the two major cellular functions in fast-growing cells. Our study suggests that bacterial chromosomes are spatially and functionally organized, analogous to eukaryotes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.