ArticleApplied and environmental microbiology2015
Phage-encoded colanic acid-degrading enzyme permits lytic phage infection of a capsule-forming resistant mutant Escherichia coli strain.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28 citing papers in PubMed, 51 citations in OpenAlex.
- Phage host range: determinants, dynamics and applications.Nature reviews. Microbiology · 2026Review
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- Genomic characterization and application on food samples of bacteriophage ETEC-S3 to control enterotoxigenic Escherichia coli.BMC research notes · 2026Article
- Phage Therapy in Plant Disease Management: 110 Years of History, Current Challenges, and Future Trends.Plants (Basel, Switzerland) · 2026Review
- Evolutionary responses of Escherichia coli to phage pressure: insights into mucoidy and colanic acid overexpression.BMC genomics · 2025Article
- A tradeoff between bacteriophage resistance and bacterial motility is mediated by the Rcs phosphorelay inMicrobiology (Reading, England) · 2024Article
- Evolutionary and co-evolutionary phage training approaches enhance bacterial suppression and delay the emergence of phage resistance.ISME communications · 2024Article
- Genome-wide phage susceptibility analysis in Acinetobacter baumannii reveals capsule modulation strategies that determine phage infectivity.PLoS pathogens · 2023Article
- Escherichia coli has robust regulatory mechanisms against elevated peptidoglycan cleavage by lytic transglycosylases.The Journal of biological chemistry · 2023Article
- Characterization and Comparative Genomic Analysis of Three VirulentInternational journal of molecular sciences · 2023Article
- Interplays of mutations inFrontiers in cellular and infection microbiology · 2023Article
- Bactericidal Effect of Cecropin A Fused Endolysin on Drug-Resistant Gram-Negative Pathogens.Journal of microbiology and biotechnology · 2022Article
- Parallel evolution ofeLife · 2022Article
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- Comparison ofViruses · 2021Article
- A SpontaneousmSphere · 2021Article
- A PolyQ Membrane Protein of Vibrio cholerae Acts as the Receptor for Phage Infection.Journal of virology · 2021Article
- Bacteriophage - A Promising Alternative Measure for Bacterial Biofilm Control.Infection and drug resistance · 2021Review
- Overproduction of Exopolysaccharide Colanic Acid by Escherichia coli by Strain Engineering and Media Optimization.Applied biochemistry and biotechnology · 2021Article
- Phage cocktail strategies for the suppression of a pathogen in a cross-feeding coculture.Microbial biotechnology · 2020Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, we isolated a bacteriophage T7-resistant mutant strain of Escherichia coli (named S3) and then proceeded to characterize it. The mutant bacterial colonies appeared to be mucoid. Microarray analysis revealed that genes related to colanic acid production were upregulated in the mutant. Increases in colanic acid production by the mutant bacteria were observed when l-fucose was measured biochemically, and protective capsule formation was observed under an electron microscope. We found a point mutation in the lon gene promoter in S3, the mutant bacterium. Overproduction of colanic acid was observed in some phage-resistant mutant bacteria after infection with other bacteriophages, T4 and lambda. Colanic acid overproduction was also observed in clinical isolates of E. coli upon phage infection. The overproduction of colanic acid resulted in the inhibition of bacteriophage adsorption to the host. Biofilm formation initially decreased shortly after infection but eventually increased after 48 h of incubation due to the emergence of the mutant bacteria. Bacteriophage PBECO4 was shown to infect the colanic acid-overproducing mutant strains of E. coli. We confirmed that the gene product of open reading frame 547 (ORF547) of PBECO4 harbored colanic acid-degrading enzymatic (CAE) activity. Treatment of the T7-resistant bacteria with both T7 and PBECO4 or its purified enzyme (CAE) led to successful T7 infection. Biofilm formation decreased with the mixed infection, too. This procedure, using a phage cocktail different from those exploiting solely receptor differences, represents a novel strategy for overcoming phage resistance in mutant bacteria.
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