ArticleApplied and environmental microbiology2015
A regulatory feedback loop between RpoS and SpoT supports the survival of Legionella pneumophila in water.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 39 citations in OpenAlex.
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- New Global Insights on the Regulation of the Biphasic Life Cycle and Virulence Via ClpP-Dependent Proteolysis in Legionella pneumophila.Molecular & cellular proteomics : MCP · 2022Article
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- Local Adaptation of Legionella pneumophila within a Hospital Hot Water System Increases Tolerance to Copper.Applied and environmental microbiology · 2021Article
- The Tail-Specific Protease Is Important for Legionella pneumophila To Survive Thermal Stress in Water and inside Amoebae.Applied and environmental microbiology · 2021Article
- Modulation of Host Lipid Pathways by Pathogenic Intracellular Bacteria.Pathogens (Basel, Switzerland) · 2020Review
- A Two-Component System That Modulates Cyclic di-GMP Metabolism Promotes Legionella pneumophila Differentiation and Viability in Low-Nutrient Conditions.Journal of bacteriology · 2019Article
- Genome-Wide Identification of Fitness Factors in Seawater forApplied and environmental microbiology · 2019Article
- Transcriptomic Changes ofFrontiers in cellular and infection microbiology · 2019Article
- The LetA/S two-component system regulates transcriptomic changes that are essential for the culturability of Legionella pneumophila in water.Scientific reports · 2018Article
- The Life Cycle ofFrontiers in cellular and infection microbiology · 2018Review
- Monitoring of nutrient limitation in growing E. coli: a mathematical model of a ppGpp-based biosensor.BMC systems biology · 2017Article
- Acyl Histidines: New N-Acyl Amides from Legionella pneumophila.Chembiochem : a European journal of chemical biology · 2017Article
- Phenotypic and Transcriptomic Responses ofFrontiers in microbiology · 2017Article
- Metabolism of myo-Inositol by Legionella pneumophila Promotes Infection of Amoebae and Macrophages.Applied and environmental microbiology · 2016Article
- Determination of viable legionellae in engineered water systems: Do we find what we are looking for?Water research · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Legionella pneumophila is a waterborne pathogen, and survival in the aquatic environment is central to its transmission to humans. Therefore, identifying genes required for its survival in water could help prevent Legionnaires' disease outbreaks. In the present study, we investigate the role of the sigma factor RpoS in promoting survival in water, where L. pneumophila experiences severe nutrient deprivation. The rpoS mutant showed a strong survival defect compared to the wild-type strain in defined water medium. The transcriptome of the rpoS mutant during exposure to water revealed that RpoS represses genes associated with replication, translation, and transcription, suggesting that the mutant fails to shut down major metabolic programs. In addition, the rpoS mutant is transcriptionally more active than the wild-type strain after water exposure. This could be explained by a misregulation of the stringent response in the rpoS mutant. Indeed, the rpoS mutant shows an increased expression of spoT and a corresponding decrease in the level of (p)ppGpp, which is due to the presence of a negative feedback loop between RpoS and SpoT. Therefore, the lack of RpoS causes an aberrant regulation of the stringent response, which prevents the induction of a successful response to starvation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.