ArticleApplied and environmental microbiology2015
Adaptation genomics of a small-colony variant in a Pseudomonas chlororaphis 30-84 biofilm.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 42 citations in OpenAlex.
- The evolution of antimicrobial peptide resistance in Pseudomonas aeruginosa is severely constrained by random peptide mixtures.PLoS biology · 2024Article
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- Evolution of cyclic di-GMP signalling on a short and long term time scale.Microbiology (Reading, England) · 2023Article
- Collateral Sensitivity to Fosfomycin of Tobramycin-Resistant Mutants ofInternational journal of molecular sciences · 2023Article
- Horizontal Gene Transfer of Antibiotic Resistance Genes in Biofilms.Antibiotics (Basel, Switzerland) · 2023Review
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- Convergent phenotypic evolution towards fosfomycin collateral sensitivity of Pseudomonas aeruginosa antibiotic-resistant mutants.Microbial biotechnology · 2022Article
- Molecular Basis of Wrinkled Variants Isolated FromFrontiers in microbiology · 2022Article
- Epiphytic Bacteria Are Essential for the Production and Transformation of Algae-Derived Carboxyl-Rich Alicyclic Molecule (CRAM)-like DOM.Microbiology spectrum · 2021Article
- Impact factors of the accumulation, migration and spread of antibiotic resistance in the environment.Environmental geochemistry and health · 2021Review
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- Genomic and transcriptomic characterization of Pseudomonas aeruginosa small colony variants derived from a chronic infection model.Microbial genomics · 2019Article
- Mutational Evolution ofFrontiers in genetics · 2018Article
- Enhanced Fluorescent Siderophore Biosynthesis and Loss of Phenazine-1-Carboxamide in Phenotypic Variant ofFrontiers in microbiology · 2018Article
- Parallel Evolution of High-Level Aminoglycoside Resistance inFrontiers in microbiology · 2018Article
- Pseudomonas chlororaphis Produces Two Distinct R-Tailocins That Contribute to Bacterial Competition in Biofilms and on Roots.Applied and environmental microbiology · 2017Article
- Draft Genome Sequence of a Chromium-Reducing Strain,Genome announcements · 2017Article
- Biostimulation and microbial community profiling reveal insights on RDX transformation in groundwater.MicrobiologyOpen · 2017Article
- Dynamics of Mutations during Development of Resistance by Pseudomonas aeruginosa against Five Antibiotics.Antimicrobial agents and chemotherapy · 2016Article
- Interspecific diversity reduces and functionally substitutes for intraspecific variation in biofilm communities.The ISME journal · 2016Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The rhizosphere-colonizing bacterium Pseudomonas chlororaphis 30-84 is an effective biological control agent against take-all disease of wheat. In this study, we characterize a small-colony variant (SCV) isolated from a P. chlororaphis 30-84 biofilm. The SCV exhibited pleiotropic phenotypes, including small cell size, slow growth and motility, low levels of phenazine production, and increased biofilm formation and resistance to antimicrobials. To better understand the genetic alterations underlying these phenotypes, RNA and whole-genome sequencing analyses were conducted comparing an SCV to the wild-type strain. Of the genome's 5,971 genes, transcriptomic profiling indicated that 1,098 (18.4%) have undergone substantial reprograming of gene expression in the SCV. Whole-genome sequence analysis revealed multiple alterations in the SCV, including mutations in yfiR (cyclic-di-GMP production), fusA (elongation factor), and cyoE (heme synthesis) and a 70-kb deletion. Genetic analysis revealed that the yfiR locus plays a major role in controlling SCV phenotypes, including colony size, growth, motility, and biofilm formation. Moreover, a point mutation in the fusA gene contributed to kanamycin resistance. Interestingly, the SCV can partially switch back to wild-type morphologies under specific conditions. Our data also support the idea that phenotypic switching in P. chlororaphis is not due to simple genetic reversions but may involve multiple secondary mutations. The emergence of these highly adherent and antibiotic-resistant SCVs within the biofilm might play key roles in P. chlororaphis natural persistence.
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