ArticleApplied and environmental microbiology2015
Competition between metals for binding to methanobactin enables expression of soluble methane monooxygenase in the presence of copper.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 32 citations in OpenAlex.
- Impact of Copper on the Physiology and Transcriptome of Methylosinus Trichosporium OB3b Grown on Either Methane or Methanol.Environmental microbiology · 2026Article
- Non-growth substrate ethane perturbs core methanotrophy in obligate methanotrophApplied and environmental microbiology · 2025Article
- Methanobactins: Structures, Biosynthesis, and Microbial Diversity.Annual review of microbiology · 2024Review
- MmoD regulates soluble methane monooxygenase and methanobactin production inApplied and environmental microbiology · 2023Article
- Article
- Variable Inhibition of Nitrous Oxide Reduction in Denitrifying Bacteria by Different Forms of Methanobactin.Applied and environmental microbiology · 2022Article
- Two TonB-Dependent Transporters in Methylosinus trichosporium OB3b Are Responsible for Uptake of Different Forms of Methanobactin and Are Involved in the Canonical "Copper Switch".Applied and environmental microbiology · 2022Article
- Evidence for methanobactin "Theft" and novel chalkophore production in methanotrophs: impact on methanotrophic-mediated methylmercury degradation.The ISME journal · 2022Article
- Chalkophores.Annual review of biochemistry · 2018Review
- Effects of copper on expression of methane monooxygenases, trichloroethylene degradation, and community structure in methanotrophic consortia.Engineering in life sciences · 2018Article
- Methanobactins: Maintaining copper homeostasis in methanotrophs and beyond.The Journal of biological chemistry · 2018Review
- Methanobactins: from genome to function.Metallomics : integrated biometal science · 2017Review
- A TonB-Dependent Transporter Is Responsible for Methanobactin Uptake by Methylosinus trichosporium OB3b.Applied and environmental microbiology · 2016Article
- Cerium regulates expression of alternative methanol dehydrogenases in Methylosinus trichosporium OB3b.Applied and environmental microbiology · 2015Article
- Methanobactin from Methylocystis sp. strain SB2 affects gene expression and methane monooxygenase activity in Methylosinus trichosporium OB3b.Applied and environmental microbiology · 2015Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is well known that copper is a key factor regulating expression of the two forms of methane monooxygenase found in proteobacterial methanotrophs. Of these forms, the cytoplasmic, or soluble, methane monooxygenase (sMMO) is expressed only at low copper concentrations. The membrane-bound, or particulate, methane monooxygenase (pMMO) is constitutively expressed with respect to copper, and such expression increases with increasing copper. Recent findings have shown that copper uptake is mediated by a modified polypeptide, or chalkophore, termed methanobactin. Although methanobactin has high specificity for copper, it can bind other metals, e.g., gold. Here we show that in Methylosinus trichosporium OB3b, sMMO is expressed and active in the presence of copper if gold is also simultaneously present. Such expression appears to be due to gold binding to methanobactin produced by M. trichosporium OB3b, thereby limiting copper uptake. Such expression and activity, however, was significantly reduced if methanobactin preloaded with copper was also added. Further, quantitative reverse transcriptase PCR (RT-qPCR) of transcripts of genes encoding polypeptides of both forms of MMO and SDS-PAGE results indicate that both sMMO and pMMO can be expressed when copper and gold are present, as gold effectively competes with copper for binding to methanobactin. Such findings suggest that under certain geochemical conditions, both forms of MMO may be expressed and active in situ. Finally, these findings also suggest strategies whereby field sites can be manipulated to enhance sMMO expression, i.e., through the addition of a metal that can compete with copper for binding to methanobactin.
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