Evidence map›Paper›PMID 25416712›Full record

ArticleRheumatology (Oxford, England)2015

Interleukin 10 hampers endothelial cell differentiation and enhances the effects of interferon α on lupus endothelial cell progenitors.

Alyssa M Cates, Victoria I Holden, Emily M Myers, Carolyne K Smith, Mariana J Kaplan, J Michelle Kahlenberg

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Rheumatology (Oxford, England), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
1.6field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 28 citations in OpenAlex.

  1. Trial
  2. Review
  3. Article
  4. Vascular damage in systemic lupus erythematosus.Nature reviews. Nephrology · 2024
    Review
  5. Article
  6. Review
  7. Review
  8. Article
  9. Article
  10. Review
  11. Article
  12. Vascular precursor cells in tissue injury repair.Translational research : the journal of laboratory and clinical medicine · 2017
    Review
  13. Review
  14. Article
  15. Article
  16. Article
  17. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Alyssa M CatesDepartment of Internal Medicine, Division of Rheumatology, Department of Microbiology and Immunology, University of Michigan, Ann Arbor, MI and Systemic Autoimmunity Branch, Intramural Research Program, National Institute of Arthritis and Musculoskeletal and Skin Disorders, National Institutes of Health, Bethesda, MD, USA.
Victoria I HoldenDepartment of Internal Medicine, Division of Rheumatology, Department of Microbiology and Immunology, University of Michigan, Ann Arbor, MI and Systemic Autoimmunity Branch, Intramural Research Program, National Institute of Arthritis and Musculoskeletal and Skin Disorders, National Institutes of Health, Bethesda, MD, USA.
Emily M MyersDepartment of Internal Medicine, Division of Rheumatology, Department of Microbiology and Immunology, University of Michigan, Ann Arbor, MI and Systemic Autoimmunity Branch, Intramural Research Program, National Institute of Arthritis and Musculoskeletal and Skin Disorders, National Institutes of Health, Bethesda, MD, USA.
Carolyne K SmithDepartment of Internal Medicine, Division of Rheumatology, Department of Microbiology and Immunology, University of Michigan, Ann Arbor, MI and Systemic Autoimmunity Branch, Intramural Research Program, National Institute of Arthritis and Musculoskeletal and Skin Disorders, National Institutes of Health, Bethesda, MD, USA.
Mariana J KaplanDepartment of Internal Medicine, Division of Rheumatology, Department of Microbiology and Immunology, University of Michigan, Ann Arbor, MI and Systemic Autoimmunity Branch, Intramural Research Program, National Institute of Arthritis and Musculoskeletal and Skin Disorders, National Institutes of Health, Bethesda, MD, USA.
J Michelle KahlenbergDepartment of Internal Medicine, Division of Rheumatology, Department of Microbiology and Immunology, University of Michigan, Ann Arbor, MI and Systemic Autoimmunity Branch, Intramural Research Program, National Institute of Arthritis and Musculoskeletal and Skin Disorders, National Institutes of Health, Bethesda, MD, USA mkahlenb@med.umich.edu.
National Institute of Arthritis and Musculoskeletal and Skin Diseases · USUniversity of Michigan–Ann Arbor · US

Funding

Systemic AutoimmunityZIAAR041199 · NIAMS · NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES · PI KAPLAN, MARIANA · 2014 to 2025
$32.5M
RESEARCH TRAINING IN EXPERIMENTAL IMMUNOPATHOLOGYT32AI007413 · NIAID · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Bethany B. Moore · 1993 to 2026
$9.8M
The Role of Innate Immunity in Systemic and Cutaneous LupusK08AR063668 · NIAMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI KAHLENBERG, JOANNE MICHELLE · 2013 to 2017
$736k
NIAID NIH HHS T32 AI007413NIAMS NIH HHS K08 AR063668
6 · The paper itself

Abstract

objectiveSLE is an autoimmune disease characterized by autoantibody generation, organ damage and an increased risk of cardiovascular disease. Generally considered an anti-inflammatory cytokine, IL-10 is increased in SLE and correlates with poor cardiovascular outcomes in the general population. The aim of this study was to explore the putative role of IL-10 in modulating endothelial function in SLE by examining the effects of this cytokine on endothelial progenitor cell/circulating angiogenic cell (EPC/CAC) differentiation.

methodsHuman and murine control and lupus EPCs/CACs were differentiated into mature endothelial cells (ECs) in the presence or absence of graded concentrations of recombinant IL-10 with or without recombinant IFN-α or a neutralizing antibody to IL-10. IL-10-deficient mice were examined to assess the role of this cytokine in type I IFN-mediated inhibition of EC differentiation and neo-angiogenesis using an in vivo Matrigel plug assay. Serum IL-10 concentrations were measured via ELISA.

resultsIL-10 hampers EC differentiation in a dose-dependent manner. In murine EPC cultures, IL-10 is required to observe the inhibitory effects of type I IFNs on EPC function and neo-angiogenesis. In human SLE EPC/CAC cultures, neutralization of IL-10 significantly improved the differentiation of EPCs, and IL-10 enhanced type I IFN-mediated EPC/CAC dysfunction. The presence of IL-10 in serum inversely correlated with EPC/CAC function in SLE but not in control cells.

conclusionIL-10 interferes with endothelial differentiation and may enhance the effects of type I IFN on vascular repair in SLE. IL-10 may be a relevant target for improving cardiovascular risk in SLE.

Indexed as

AdultAnimalsCell DifferentiationEndothelial Progenitor CellsEnzyme-Linked Immunosorbent AssayFemaleHumansInterferon-alphaInterleukin-10Lupus Erythematosus, SystemicMaleMiceMice, Inbred BALB CMiddle AgedInterferon-alphaInterleukin-10cardiovascularendothelial progenitorIL-10interferon αlupus

Identifiers

PMID25416712
PMCPMC4447841
OpenAlexW2097320195

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.