ArticleMethods in enzymology2014
Imaging changes in the cytosolic ATP-to-ADP ratio.
Article in Methods in enzymology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 32 citations in OpenAlex.
- ATP-Red enables single-cell bioenergetic phenotyping by flow cytometry.Cell reports methods · 2026Article
- Intracellular lactate dynamics iniScience · 2025Article
- Variable bioenergetic sensitivity of neurons and astrocytes to insulin and extracellular glucose.npj metabolic health and disease · 2024Article
- Calculation of ATP production rates using the Seahorse XF Analyzer.EMBO reports · 2023Article
- Mechanistic multiscale modelling of energy metabolism in human astrocytes reveals the impact of morphology changes in Alzheimer's Disease.PLoS computational biology · 2023Article
- Dietary ribose supplementation improves flesh quality through purine metabolism in gibel carp (Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2023Article
- Considerations for using isolated cell systems to understand cardiac metabolism and biology.Journal of molecular and cellular cardiology · 2021Review
- Exposure to Trichloroethylene MetaboliteChemical research in toxicology · 2020Article
- Genetically Engineered Human Kidney Cells for Real-Time Cytotoxicity Testing In Vitro.Molecular biotechnology · 2020Article
- Article
- Excess hydrogen sulfide and polysulfides production underlies a schizophrenia pathophysiology.EMBO molecular medicine · 2019Article
- Fast bioelectrical switches.Nature chemical biology · 2019Article
- Preserving Insulin Secretion in Diabetes by Inhibiting VDAC1 Overexpression and Surface Translocation in β Cells.Cell metabolism · 2019Article
- Minimizing ATP depletion by oxygen scavengers for single-molecule fluorescence imaging in live cells.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Regulation of ATP utilization during metastatic cell migration by collagen architecture.Molecular biology of the cell · 2018Article
- Use of luciferase probes to measure ATP in living cells and animals.Nature protocols · 2017Article
- A simple in vitro tumor chemosensitivity assay based on cell penetrating peptide tagged luciferase.PloS one · 2017Article
- Practical guide for dynamic monitoring of protein oxidation using genetically encoded ratiometric fluorescent biosensors of methionine sulfoxide.Methods (San Diego, Calif.) · 2016Review
- Imaging Adenosine Triphosphate (ATP).The Biological bulletin · 2016Review
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Adenosine triphosphate (ATP) is a central metabolite that plays fundamental roles as an energy transfer molecule, a phosphate donor, and a signaling molecule inside the cells. The phosphoryl group transfer potential of ATP provides a thermodynamic driving force for many metabolic reactions, and phosphorylation of both small metabolites and large proteins can serve as a regulatory modification. In the process of phosphoryl transfer from ATP, the diphosphate ADP is produced, and as a result, the ATP-to-ADP ratio is an important physiological control parameter. The ATP-to-ADP ratio is directly proportional to cellular energy charge and phosphorylation potential. Furthermore, several ATP-dependent enzymes and signaling proteins are regulated by ADP, and their activation profiles are a function of the ATP-to-ADP ratio. Finally, regeneration of ATP from ADP can serve as an important readout of energy metabolism and mitochondrial function. We, therefore, developed a genetically encoded fluorescent biosensor tuned to sense ATP-to-ADP ratios in the physiological range of healthy mammalian cells. Here, we present a protocol for using this biosensor to visualize energy status using live-cell fluorescence microscopy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.