ArticleEukaryotic cell2015
Ctk1 function is necessary for full translation initiation activity in Saccharomyces cerevisiae.
Article in Eukaryotic cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
- Absence of CDK12 in oocyte leads to female infertility.Cell death & disease · 2025Article
- Physiological and pathological roles of the transcriptional kinases CDK12 and CDK13 in the central nervous system.Cell death and differentiation · 2025Review
- Cyclin-dependent kinases: Masters of the eukaryotic universe.Wiley interdisciplinary reviews. RNA · 2023Review
- Coming of Age: Targeting Cyclin K in Cancers.Cells · 2023Review
- Article
- Structure and activation mechanism of the yeast RNA Pol II CTD kinase CTDK-1 complex.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Lithium Chloride Sensitivity in Yeast and Regulation of Translation.International journal of molecular sciences · 2020Article
- Gene expression regulation by CDK12: a versatile kinase in cancer with functions beyond CTD phosphorylation.Experimental & molecular medicine · 2020Review
- A SUMO-dependent pathway controls elongating RNA Polymerase II upon UV-induced damage.Scientific reports · 2019Article
- CDK12 Activity-Dependent Phosphorylation Events in Human Cells.Biomolecules · 2019Article
- Multiple roles of CTDK-I throughout the cell.Cellular and molecular life sciences : CMLS · 2019Review
- CDK12 phosphorylates 4E-BP1 to enable mTORC1-dependent translation and mitotic genome stability.Genes & development · 2019Article
- Screening for functional IRESes using α-complementation system of β-galactosidase inBiotechnology for biofuels · 2019Article
- P-TEFb Kinase Activity Is Essential for Global Transcription, Resumption of Meiosis and Embryonic Genome Activation in Pig.PloS one · 2016Article
- Nuclear export of messenger RNA.Genes · 2015Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Translation is a fundamental and highly regulated cellular process. Previously, we reported that the kinase and transcription elongation factor Ctk1 increases fidelity during translation elongation in Saccharomyces cerevisiae. Here, we show that loss of Ctk1 function also affects the initiation step of translation. Translation active extracts from Ctk1-depleted cells show impaired translation activity of capped mRNA, but not mRNA reporters containing the cricket paralysis virus (CrPV) internal ribosome entry site (IRES). Furthermore, the formation of 80S initiation complexes is decreased, which is probably due to reduced subunit joining. In addition, we determined the changes in the phosphorylation pattern of a ribosome enriched fraction after depletion of Ctk1. Thus, we provide a catalogue of phosphoproteomic changes dependent on Ctk1. Taken together, our data suggest a stimulatory function of Ctk1 in 80S formation during translation initiation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.