ArticleThe Plant cell2014
The microtubule plus-end tracking proteins SPR1 and EB1b interact to maintain polar cell elongation and directional organ growth in Arabidopsis.
Article in The Plant cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 53 citations in OpenAlex.
- Cytoskeletal control of development and environmental adaptation in plants.Science China. Life sciences · 2026Review
- The epidermis coordinates multi-scale symmetry breaking in chiral root growth.Nature communications · 2025Article
- Transverse cortical microtubule arrays form persistent unipolar domains in hypocotyl cells ofMolecular biology of the cell · 2025Article
- FERONIA adjusts CC1 phosphorylation to control microtubule array behavior in response to salt stress.Science advances · 2024Article
- Endomembrane trafficking driven by microtubule growth regulates stomatal movement in Arabidopsis.Nature communications · 2024Article
- The Microtubule End Binding Protein Mal3 Is Essential for the Dynamic Assembly of Microtubules duringInternational journal of molecular sciences · 2024Article
- Anatomical observation and transcriptome analysis of branch-twisted mutations in Chinese jujube.BMC genomics · 2023Article
- PHYTOCHROME INTERACTING FACTOR 4 regulates microtubule organization to mediate high temperature-induced hypocotyl elongation in Arabidopsis.The Plant cell · 2023Article
- Genome-Wide Identification, Characterization, and Expression Analysis ofInternational journal of molecular sciences · 2023Article
- The OPEN STOMATA1-SPIRAL1 module regulates microtubule stability during abscisic acid-induced stomatal closure in Arabidopsis.The Plant cell · 2023Article
- Imaging the living plant cell: From probes to quantification.The Plant cell · 2022Review
- The regulation of the cell wall by glycosylphosphatidylinositol-anchored proteins inFrontiers in cell and developmental biology · 2022Review
- Plant Gravitropism: From Mechanistic Insights into Plant Function on Earth to Plants Colonizing Other Worlds.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Article
- Analysis of formin functions during cytokinesis using specific inhibitor SMIFH2.Plant physiology · 2021Article
- Microtubule self-organisation during seed germination in Arabidopsis.BMC biology · 2020Article
- Complementary Superresolution Visualization of Composite Plant Microtubule Organization and Dynamics.Frontiers in plant science · 2020Article
- Root Skewing-Associated Genes Impact the Spaceflight Response ofFrontiers in plant science · 2020Article
- Mechanism of microtubule plus-end tracking by the plant-specific SPR1 protein and its development as a versatile plus-end marker.The Journal of biological chemistry · 2019Article
- CLASP stabilization of plus ends created by severing promotes microtubule creation and reorientation.The Journal of cell biology · 2019Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
The microtubule plus-end tracking proteins (+TIPs) END BINDING1b (EB1b) and SPIRAL1 (SPR1) are required for normal cell expansion and organ growth. EB proteins are viewed as central regulators of +TIPs and cell polarity in animals; SPR1 homologs are specific to plants. To explore if EB1b and SPR1 fundamentally function together, we combined genetic, biochemical, and cell imaging approaches in Arabidopsis thaliana. We found that eb1b-2 spr1-6 double mutant roots exhibit substantially more severe polar expansion defects than either single mutant, undergoing right-looping growth and severe axial twisting instead of waving on tilted hard-agar surfaces. Protein interaction assays revealed that EB1b and SPR1 bind each other and tubulin heterodimers, which is suggestive of a microtubule loading mechanism. EB1b and SPR1 show antagonistic association with microtubules in vitro. Surprisingly, our combined analyses revealed that SPR1 can load onto microtubules and function independently of EB1 proteins, setting SPR1 apart from most studied +TIPs in animals and fungi. Moreover, we found that the severity of defects in microtubule dynamics in spr1 eb1b mutant hypocotyl cells correlated well with the severity of growth defects. These data indicate that SPR1 and EB1b have complex interactions as they load onto microtubule plus ends and direct polar cell expansion and organ growth in response to directional cues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.