ArticleJournal of biomedical optics2015
Comparing in vivo pump-probe and multiphoton fluorescence microscopy of melanoma and pigmented lesions.
Article in Journal of biomedical optics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed.
- Angle-resolved Measurements Reveal the Origin of Signal Anisotropy in Pump-probe Microscopy.bioRxiv : the preprint server for biology · 2025Article
- Recent Advances in Characterization of Melanin Pigments in Biological Samples.International journal of molecular sciences · 2023Review
- In vivo melanin 3D quantification and z-epidermal distribution by multiphoton FLIM, phasor and Pseudo-FLIM analyses.Scientific reports · 2022Article
- Label-Free Delineation of Human Uveal Melanoma Infiltration With Pump-Probe Microscopy.Frontiers in oncology · 2022Article
- Melanins as Sustainable Resources for Advanced Biotechnological Applications.Global challenges (Hoboken, NJ) · 2021Review
- Melanin distribution from the dermal-epidermal junction to the stratum corneum: non-invasive in vivo assessment by fluorescence and Raman microspectroscopy.Scientific reports · 2020Article
- Analysis of Melanin Structure and Biochemical Composition in Conjunctival Melanocytic Lesions Using Pump-Probe Microscopy.Translational vision science & technology · 2019Article
- Unraveling the molecular nature of melanin changes in metastatic cancer.Journal of biomedical optics · 2019Article
- Comparison of pump-probe and hyperspectral imaging in unstained histology sections of pigmented lesions.Biomedical optics express · 2017Article
- Label-Free Imaging of Female Genital Tract Melanocytic Lesions With Pump-Probe Microscopy: A Promising Diagnostic Tool.Journal of lower genital tract disease · 2017Article
- In vivo coherent Raman imaging of the melanomagenesis-associated pigment pheomelanin.Scientific reports · 2016Article
- Dual-wavelength pump-probe microscopy analysis of melanin composition.Scientific reports · 2016Article
- Invited Review Article: Pump-probe microscopy.The Review of scientific instruments · 2016Review
- Flexible digital signal processing architecture for narrowband and spread-spectrum lock-in detection in multiphoton microscopy and time-resolved spectroscopy.The Review of scientific instruments · 2015Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
We demonstrate a multimodal approach that combines a pump-probe with confocal reflectance and multiphoton autofluorescence microscopy. Pump-probe microscopy has been proven to be of great value in analyzing thin tissue sections of pigmented lesions, as it produces molecular contrast which is inaccessible by other means. However, the higher optical intensity required to overcome scattering in thick tissue leads to higher-order nonlinearities in the optical response of melanin (e.g., two-photon pump and one-photon probe) that present additional challenges for interpreting the data. We show that analysis of pigment composition in vivo must carefully account for signal terms that are nonlinear with respect to the pump and probe intensities. We find that pump-probe imaging gives useful contrast for pigmented structures over a large range of spatial scales (100 μm to 1 cm), making it a potentially useful tool for tracking the progression of pigmented lesions without the need to introduce exogenous contrast agents.
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Registered trials
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