ArticlePloS one2014
Transcript profiling reveals auxin and cytokinin signaling pathways and transcription regulation during in vitro organogenesis of Ramie (Boehmeria nivea L. Gaud).
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Improving Ramie Fiber: Current Progress and Future Directions in Molecular Breeding.Plants (Basel, Switzerland) · 2026Review
- Chloroplast genome characteristics and phylogenetic analysis of Ramie (Boehmeria nivea (L.) Gaudich).Molecular biology reports · 2026Article
- Physiological, Metabolic, and Transcriptomic Analyses Reveal Mechanisms of Proliferation and Somatic Embryogenesis of Litchi (International journal of molecular sciences · 2024Article
- An Introduction to Plant Cell, Tissue, and Organ Culture: Current Status and Perspectives.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Transcriptome Sequencing ofPlants (Basel, Switzerland) · 2023Article
- Identification and characterization of microRNAs in phloem and xylem from ramie (Boehmeria nivea).Molecular biology reports · 2020Article
- PromoterFrontiers in genetics · 2020Article
- Efficient Characterization of Tetraploid Watermelon.Plants (Basel, Switzerland) · 2019Article
- De Novo Transcriptome Assembly ofGenes · 2019Article
- PtWOX11 acts as master regulator conducting the expression of key transcription factors to induce de novo shoot organogenesis in poplar.Plant molecular biology · 2018Article
- Identification and expression characterization of the Phloem Protein 2 (PP2) genes in ramie (Boehmeria nivea L. Gaudich).Scientific reports · 2018Article
- Transcriptome Comparison Reveals Distinct Selection Patterns in Domesticated and Wild Agave Species, the Important CAM Plants.International journal of genomics · 2018Article
- De novo assembly and comparative analysis of the transcriptome of embryogenic callus formation in bread wheat (Triticum aestivum L.).BMC plant biology · 2017Article
- QTL analysis of four main stem bark traits using a GBS-SNP-based high-density genetic map in ramie.Scientific reports · 2017Article
- Genome-wide identification of SAUR genes in watermelon (Physiology and molecular biology of plants : an international journal of functional plant biology · 2017Article
- Article
- Transcriptomic and Hormonal Analyses Reveal that YUC-Mediated Auxin Biogenesis Is Involved in Shoot Regeneration from Rhizome inFrontiers in plant science · 2017Article
- Identification of small auxin-up RNA (SAUR) genes in Urticales plants: mulberry (Morus notabilis), hemp (Cannabis sativa) and ramie (Boehmeria nivea).Journal of genetics · 2016Article
- Transcriptome Analysis of the Hepatopancreas in the Pacific White Shrimp (Litopenaeus vannamei) under Acute Ammonia Stress.PloS one · 2016Article
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9 authors.
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Abstract
In vitro organogenesis, one of the most common pathways leading to in vitro plant regeneration, is widely used in biotechnology and the fundamental study of plant biology. Although previous studies have constructed a complex regulatory network model for Arabidopsis in vitro organogenesis, no related study has been reported in ramie. To generate more complete observations of transcriptome content and dynamics during ramie in vitro organogenesis, we constructed a reference transcriptome library and ten digital gene expression (DGE) libraries for illumina sequencing. Approximately 111.34 million clean reads were obtained for transcriptome and the DGE libraries generated between 13.5 and 18.8 million clean reads. De novo assembly produced 43,222 unigenes and a total of 5,760 differentially expressed genes (DEGs) were filtered. Searching against the Kyoto Encyclopedia of Genes and Genomes Pathway database, 26 auxin related and 11 cytokinin related DEGs were selected for qRT-PCR validation of two ramie cultivars, which had high (Huazhu No. 5) or extremely low (Dazhuhuangbaima) shoot regeneration abilities. The results revealed differing regulation patterns of auxin and cytokinin in different genotypes. Here we report the first genome-wide gene expression profiling of in vitro organogenesis in ramie and provide an overview of transcription and phytohormone regulation during the process. Furthermore, the auxin and cytokinin related genes have distinct expression patterns in two ramie cultivars with high or extremely low shoot regeneration ability, which has given us a better understanding of the in vitro organogenesis mechanism. This result will provide a foundation for future phytohormone research and lead to improvements of the ramie regeneration system.
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