Evidence map›Paper›PMID 25415276›Full record

ArticlePlastic and reconstructive surgery2015

Stem cells rejuvenate radiation-impaired vasculogenesis in murine distraction osteogenesis.

Sagar S Deshpande, Kathleen K Gallagher, Alexis Donneys, Noah S Nelson, Nicholas P Guys, Peter A Felice, Erin E Page, Hongli Sun, Paul H Krebsbach, Steven R Buchman

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plastic and reconstructive surgery, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed, 1 pooled it
2.3field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 1 synthesis or guideline pooled it, 45 citations in OpenAlex.

  1. Pooled it
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  14. A Histomorphometric Analysis of Radiation Damage in an Isogenic Murine Model of Distraction Osteogenesis.Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons · 2015
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 2 countries.

Sagar S DeshpandeAnn Arbor, Mich.; and Charleston, S.C. From the Craniofacial Research Laboratory, the Department of Otolaryngology, and the Section of Plastic Surgery, Department of Surgery, University of Michigan Health System; the University of Michigan Medical School; the College of Literature, Sciences, and the Arts, University of Michigan; the Division of General Surgery, Department of Surgery, Medical University of South Carolina; and the Department of Biologic and Materials Sciences, University of Michigan Dental School.
Kathleen K Gallagher
Alexis Donneys
Noah S Nelson
Nicholas P Guys
Peter A Felice
Erin E Page
Hongli Sun
Paul H Krebsbach
Steven R Buchman
University of Michigan–Ann Arbor · USAnn Arbor Center for Independent Living · USBio-Medical Science (South Korea) · KRMedical University of South Carolina · USUniversity of Charleston · US

Funding

TRAINING GRANT IN TRAUMA, BURN AND WOUND HEALING RESEARCT32GM008616 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI BUCHMAN, STEVEN R, CHUNG, KEVIN C · 1997 to 2025
$3.1M
Translational Optimization of Bone Regeneration in the Irradiated MandibleR01CA125187 · NCI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI BUCHMAN, STEVEN R · 2007 to 2017
$2.8M
NCI NIH HHS R01 CA125187NCI NIH HHS R01 CA 125187-06NIGMS NIH HHS T32 GM 008616NIGMS NIH HHS T32 GM008616
6 · The paper itself

Abstract

backgroundRadiotherapy is known to be detrimental to bone and soft-tissue repair. Bone marrow stromal cells have been shown to enhance bone regeneration during distraction osteogenesis following radiation therapy. The authors posit that transplanted bone marrow stromal cells will significantly augment the mandibular vascularity devastated by radiation therapy.

methodsNineteen male Lewis rats were split randomly into three groups: distraction osteogenesis only (n = 5), radiation therapy plus distraction osteogenesis (n = 7), and radiation therapy plus distraction osteogenesis with intraoperative placement of 2 million bone marrow stromal cells (n = 7). A mandibular osteotomy was performed, and an external fixator device was installed. From postoperative days 4 through 12, rats underwent a gradual 5.1-mm distraction followed by a 28-day consolidation period. On postoperative day 40, Microfil was perfused into the vasculature and imaging commenced. Vascular radiomorphometric values were calculated for regions of interest. An analysis of variance with post hoc Tukey or Games-Howell tests was used, dependent on data homogeneity.

resultsStereologic analysis indicated significant remediation in vasculature in the bone marrow stromal cell group compared with the radiation therapy/distraction osteogenesis group. Each of five metrics idicated significant improvements from radiation therapy/distraction osteogenesis to the bone marrow stromal cell group, with no difference between the bone marrow stromal cell group and the distraction osteogenesis group.

conclusionsBone marrow stromal cells used together with distraction osteogenesis can rejuvenate radiation-impaired vasculogenesis in the mandible, reversing radiation therapy-induced isotropy and creating a robust vascular network. Bone marrow stromal cells may offer clinicians an alternative reconstructive modality that could improve the lifestyle of patients with hypovascular bone.

Indexed as

Bone RegenerationAnimalsBone Marrow CellsMaleMandibleNeovascularization, PhysiologicOsteogenesis, DistractionOsteoradionecrosisRadiation Injuries, ExperimentalRatsRats, Inbred LewStem Cell Transplantation

Identifiers

PMID25415276
PMCPMC4608845
OpenAlexW2053277586

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.