ArticleeLife2014
Fast retrieval and autonomous regulation of single spontaneously recycling synaptic vesicles.
Article in eLife, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 55 citations in OpenAlex.
- Formation, Organization, and Maintenance of Synaptic Vesicle Pools: From Molecules to Structure and Dynamics.Advances in neurobiology · 2026Review
- Lysophosphatidic acid selectively modulates excitatory transmission in hippocampal neurons.Cell & bioscience · 2025Article
- Spatially non-overlapping CaCell reports · 2023Article
- Cellular and subcellular optogenetic approaches towards neuroprotection and vision restoration.Progress in retinal and eye research · 2023Review
- Two forms of asynchronous release with distinctive spatiotemporal dynamics in central synapses.eLife · 2023Article
- Exploiting the molecular diversity of the synapse to investigate neuronal communication: A guide through the current toolkit.The European journal of neuroscience · 2022Review
- MeCP2 loss-of-function dysregulates microRNAs regionally and disrupts excitatory/inhibitory synaptic transmission balance.Hippocampus · 2022Article
- Article
- Presynaptic mechanisms underlying GABACell reports · 2022Article
- Effects of the clathrin inhibitor Pitstop-2 on synaptic vesicle recycling at a central synapseFrontiers in synaptic neuroscience · 2022Article
- Methods of measuring presynaptic function with fluorescence probes.Applied microscopy · 2021Review
- Single synapse glutamate imaging reveals multiple levels of release mode regulation in mammalian synapses.iScience · 2021Article
- Myosin V Regulates Spatial Localization of Different Forms of Neurotransmitter Release in Central Synapses.Frontiers in synaptic neuroscience · 2021Article
- An ultrasensitive biosensor for high-resolution kinase activity imaging in awake mice.Nature chemical biology · 2021Article
- VAMP4 Maintains a CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Spontaneous and evoked neurotransmission are partially segregated at inhibitory synapses.eLife · 2020Article
- Zebrafish neuromuscular junction: The power of N.Neuroscience letters · 2019Review
- The Synaptic Vesicle Cycle Revisited: New Insights into the Modes and Mechanisms.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Review
- Presynaptic origins of distinct modes of neurotransmitter release.Current opinion in neurobiology · 2018Review
- Copine-6 Binds to SNAREs and Selectively Suppresses Spontaneous Neurotransmission.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Presynaptic terminals release neurotransmitters spontaneously in a manner that can be regulated by Ca(2+). However, the mechanisms underlying this regulation are poorly understood because the inherent stochasticity and low probability of spontaneous fusion events has curtailed their visualization at individual release sites. Here, using pH-sensitive optical probes targeted to synaptic vesicles, we visualized single spontaneous fusion events and found that they are retrieved extremely rapidly with faster re-acidification kinetics than their action potential-evoked counterparts. These fusion events were coupled to postsynaptic NMDA receptor-driven Ca(2+) signals, and at elevated Ca(2+) concentrations there was an increase in the number of vesicles that would undergo fusion. Furthermore, spontaneous vesicle fusion propensity in a synapse was Ca(2+)-dependent but regulated autonomously: independent of evoked fusion probability at the same synapse. Taken together, these results expand classical quantal analysis to incorporate endocytic and exocytic phases of single fusion events and uncover autonomous regulation of spontaneous fusion.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.