ArticleAvicenna journal of medical biotechnology2014
Mitochondrial Distribution and ATP Content of Vitrified, In vitro Matured Mouse Oocytes.
Article in Avicenna journal of medical biotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 31 citations in OpenAlex.
- Vitrification altered αV and β3 integrin expression in mouse oocytes in a maturation stage-dependent manner.Molecular biology reports · 2026Article
- The Effect of Edaravone Supplementation Prior to Cryopreservation on Sperm Parameters, DNA Integrity, Apoptosis, Lipid Peroxidation, and Mitochondrial Membrane Potential in Infertile Men with Asthenoteratozoospermia.Reproductive sciences (Thousand Oaks, Calif.) · 2025Article
- Prenatal Silicon Dioxide Nanoparticles Exposure Reduces Female Offspring Fertility Without Affecting Males.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Fructose improves titanium dioxide nanoparticles induced alterations in developmental competence of mouse oocytes.BMC veterinary research · 2024Article
- Glutathione during Post-Thaw Recovery Culture Can Mitigate Deleterious Impact of Vitrification on Bovine Oocytes.Antioxidants (Basel, Switzerland) · 2022Article
- Preserving Oocytes in Oncofertility†.Biology of reproduction · 2022Review
- Effect of vitrification on global gene expression dynamics of bovine elongating embryos.Reproduction, fertility, and development · 2021Article
- Highly successful production of viable mice derived from vitrified germinal vesicle oocytes.PloS one · 2021Article
- In Vitro Maturation with Leukemia Inhibitory Factor Prior to the Vitrification of Bovine Oocytes Improves Their Embryo Developmental Potential and Gene Expression in Oocytes and Embryos.International journal of molecular sciences · 2020Article
- Blastocyst mitochondrial DNA (mtDNA) is not affected by oocyte vitrification: a sibling oocyte study.Journal of assisted reproduction and genetics · 2020Article
- Article
- N-acetylcysteine addition after vitrification improves oocyte mitochondrial polarization status and the quality of embryos derived from vitrified murine oocytes.BMC veterinary research · 2019Article
- Calcium sensing receptor protects high glucose-induced energy metabolism disorder via blocking gp78-ubiquitin proteasome pathway.Cell death & disease · 2017Article
- Nutrient deprivation-related OXPHOS/glycolysis interconversion via HIF-1α/C-MYC pathway in U251 cells.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- Vitrification of Mouse MII Oocyte Decreases the Mitochondrial DNA Copy Number, TFAM Gene Expression and Mitochondrial Enzyme Activity.Journal of reproduction & infertilityArticle
- The Effect of Preincubation Time and Myo-inositol Supplementation on the Quality of Mouse MII Oocytes.Journal of reproduction & infertilityArticle
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe objective of this study was to investigate the effect of vitrification and in vitro maturation on the mitochondrial distribution and ATP content of oocytes.
methodsThe oocytes at Germinal Vesicle (GV) and Metaphase II (MII) stages were recovered from 6-8 week old NMRI strain female mice. The oocytes were divided into vitrified and non-vitrified groups. Vitrification was done by the cryotop method using ethylene glycol, dimethylsulfoxide and sucrose as cryoprotectants. The GV oocytes were cultured in maturation medium for 24 hrs. The collected in vitro matured oocytes (IVM-MII) and ovulated metaphase II (OV-MII) oocytes were inseminated with capacitated sperm. The ATP content of the oocytes was measured by luciferin-luciferase reaction. Distribution of oocyte mitochondria was studied using Mito Tracker Green staining under fluorescent microscope.
resultsThe survival rates of vitrified oocytes at GV and MII stages were 87.39 and 89.5%, respectively. There was no significant difference in the developmental and hatching rates of vitrified and non-vitrified oocytes. The ATP content of GV and MII oocytes derived from in vivo and in vitro condition was not significantly different in vitrified and non-vitrified samples. The pattern of mitochondrial distribution in vitrified and non-vitrified GV and MII oocytes was similar but it was different between MII oocytes collected from fallopian tube and in vitro matured MII oocytes. However, the florescent intensity of mitochondrial staining was different in all the groups in the study.
conclusionVitrification did not affect mouse oocyte developmental competence, ATP content at different developmental stages but some alteration was seen in mitochondria distribution of in vitro matured oocytes in comparison to their controls.
Indexed as
Identifiers
25414783PMC4224660W2139222386What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.