ArticleCardiovascular engineering and technology2013
Gentamicin Reduces Calcific Nodule Formation by Aortic Valve Interstitial Cells
Article in Cardiovascular engineering and technology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed, 8 citations in OpenAlex.
- Mapping regulators of cell fate determination: Approaches and challenges.APL bioengineering · 2020Review
- Dysregulation of hyaluronan homeostasis during aortic valve disease.Matrix biology : journal of the International Society for Matrix Biology · 2017Article
- Ascorbic acid promotes extracellular matrix deposition while preserving valve interstitial cell quiescence within 3D hydrogel scaffolds.Journal of tissue engineering and regenerative medicine · 2017Article
- Effects of Raloxifene on the Proliferation and Apoptosis of Human Aortic Valve Interstitial Cells.BioMed research international · 2016Article
- Multilayer three-dimensional filter paper constructs for the culture and analysis of aortic valvular interstitial cells.Acta biomaterialia · 2015Article
- Differential Aortic and Mitral Valve Interstitial Cell Mineralization and the Induction of Mineralization by LysophosphatidylcholineCardiovascular engineering and technology · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
purposeGentamicin is a widely employed antibiotic, but may reduce calcium uptake by eukaryotic cells. This study was conducted to determine whether gentamicin reduces calcification by porcine aortic valvular interstitial cells (pAVICs) grown in 2D culture, which is a common model for calcific aortic valve disease (CAVD). METHODS AND
resultsThe presence of gentamicin (up to 0.2 mM) in the medium of pAVICs cultured for 8 days significantly lowered calcification and alkaline phosphatase content in a dose-dependent manner compared to pAVICs cultured without gentamicin. Gentamicin also significantly increased cell proliferation and apoptosis at concentrations of 0.1-0.2 mM. Next, gentamicin was applied to previously calcified pAVIC cultures (grown for 8 days) to determine whether it could stop or reverse the calcification process. Daily application of gentamicin for 8 additional days significantly reduced calcification to below the pre-calcification levels.
conclusionsThese results confirm that gentamicin should be used cautiously with
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.