ArticleNucleic acids research2014
The TCF C-clamp DNA binding domain expands the Wnt transcriptome via alternative target recognition.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- Differential Wnt/β-catenin signaling via TCF7L2/LEF1 binding specificity shapes cellular and tumor phenotypes.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Perspectives on Codebook: sequence specificity of uncharacterized human transcription factors.bioRxiv : the preprint server for biology · 2024Article
- TCF7L1 regulates colorectal cancer cell migration by repressing GAS1 expression.Scientific reports · 2024Article
- Circadian clocks in health and disease: Dissecting the roles of the biological pacemaker in cancer.F1000Research · 2023Review
- A new CUT&RUN low volume-urea (LoV-U) protocol optimized for transcriptional co-factors uncovers Wnt/β-catenin tissue-specific genomic targets.Development (Cambridge, England) · 2022Article
- Disruption of the circadian clock drivesScience advances · 2022Article
- Wnt target enhancer regulation by a CDX/TCF transcription factor collective and a novel DNA motif.Nucleic acids research · 2021Article
- Article
- Loss of the nuclear Wnt pathway effector TCF7L2 promotes migration and invasion of human colorectal cancer cells.Oncogene · 2020Article
- Loss of MBNL1 induces RNA misprocessing in the thymus and peripheral blood.Nature communications · 2020Article
- The Impact of High-mobility Group Box Mutation of T-cell Factor 4 on Its Genomic Binding Pattern in Non-small Cell Lung Cancer.Translational oncology · 2020Article
- Developmentally regulatedeLife · 2019Article
- Orthogonal γPNA Dimerization Domains Empower DNA Binders with Cooperativity and Versatility Mimicking that of Transcription Factor Pairs.Chemistry (Weinheim an der Bergstrasse, Germany) · 2018Article
- Wnt/Tcf1 pathway restricts embryonic stem cell cycle through activation of the Ink4/Arf locus.PLoS genetics · 2017Article
- A new mode of DNA binding distinguishes Capicua from other HMG-box factors and explains its mutation patterns in cancer.PLoS genetics · 2017Article
- Wnt target genes and where to find them.F1000Research · 2017Review
- Topoisomerase IIα mediates TCF-dependent epithelial-mesenchymal transition in colon cancer.Oncogene · 2016Article
- Review
- Review
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Authors and funding
9 authors.
Funding
Abstract
LEF/TCFs direct the final step in Wnt/β-catenin signalling by recruiting β-catenin to genes for activation of transcription. Ancient, non-vertebrate TCFs contain two DNA binding domains, a High Mobility Group box for recognition of the Wnt Response Element (WRE; 5'-CTTTGWWS-3') and the C-clamp domain for recognition of the GC-rich Helper motif (5'-RCCGCC-3'). Two vertebrate TCFs (TCF-1/TCF7 and TCF-4/TCF7L2) use the C-clamp as an alternatively spliced domain to regulate cell-cycle progression, but how the C-clamp influences TCF binding and activity genome-wide is not known. Here, we used a doxycycline inducible system with ChIP-seq to assess how the C-clamp influences human TCF1 binding genome-wide. Metabolic pulse-labeling of nascent RNA with 4'Thiouridine was used with RNA-seq to connect binding to the Wnt transcriptome. We find that the C-clamp enables targeting to a greater number of gene loci for stronger occupancy and transcription regulation. The C-clamp uses Helper sites concurrently with WREs for gene targeting, but it also targets TCF1 to sites that do not have readily identifiable canonical WREs. The coupled ChIP-seq/4'Thiouridine-seq analysis identified new Wnt target genes, including additional regulators of cell proliferation. Thus, C-clamp containing isoforms of TCFs are potent transcriptional regulators with an expanded transcriptome directed by C-clamp-Helper site interactions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.