ArticleJournal of cell science2015
Tyrosine phosphorylation of WIP releases bound WASP and impairs podosome assembly in macrophages.
Article in Journal of cell science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
- Plasticity in leukocyte migration during haematopoiesis and inflammation.Journal of muscle research and cell motility · 2025Review
- Review
- When Actin is Not Actin' Like It Should: A New Category of Distinct Primary Immunodeficiency Disorders.Journal of innate immunity · 2021Review
- Molecular Mechanisms of Leukocyte Migration and Its Potential Targeting-Lessons Learned From MKL1/SRF-Related Primary Immunodeficiency Diseases.Frontiers in immunology · 2021Review
- Elucidating the Biomechanics of Leukocyte Transendothelial Migration by Quantitative Imaging.Frontiers in cell and developmental biology · 2021Review
- Combined AFM and super-resolution localisation microscopy: Investigating the structure and dynamics of podosomes.European journal of cell biology · 2020Article
- The Disordered Cellular Multi-Tasker WIP and Its Protein-Protein Interactions: A Structural View.Biomolecules · 2020Review
- Article
- Costameres, dense plaques and podosomes: the cell matrix adhesions in cardiovascular mechanosensing.Journal of muscle research and cell motility · 2019Review
- The Rényi divergence enables accurate and precise cluster analysis for localization microscopy.Bioinformatics (Oxford, England) · 2018Article
- Artifact-free high-density localization microscopy analysis.Nature methods · 2018Article
- Podosome assembly is controlled by the GTPase ARF1 and its nucleotide exchange factor ARNO.The Journal of cell biology · 2017Article
- WIP and WICH/WIRE co-ordinately control invadopodium formation and maturation in human breast cancer cell invasion.Scientific reports · 2016Article
- Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
Podosomes are integrin-containing adhesion structures commonly found in migrating leukocytes of the monocytic lineage. The actin cytoskeletal organisation of podosomes is based on a WASP- and Arp2/3-mediated mechanism. WASP also associates with a second protein, WIP (also known as WIPF1), and they co-localise in podosome cores. Here, we report for the first time that WIP can be phosphorylated on tyrosine residues and that tyrosine phosphorylation of WIP is a trigger for release of WASP from the WIP-WASP complex. Using a knockdown approach together with expression of WIP phosphomimics, we show that in the absence of WIP-WASP binding, cellular WASP is rapidly degraded, leading to disruption of podosomes and a failure of cells to degrade an underlying matrix. In the absence of tyrosine phosphorylation, the WIP-WASP complex remains intact and podosome lifetimes are extended. A screen of candidate kinases and inhibitor-based assays identified Bruton's tyrosine kinase (Btk) as a regulator of WIP tyrosine phosphorylation. We conclude that tyrosine phosphorylation of WIP is a crucial regulator of WASP stability and function as an actin-nucleation-promoting factor.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.