ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2015
High-resolution genetic mapping and physical map construction for the fertility restorer Rfm1 locus in barley.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Genome-wide analysis ofFrontiers in plant science · 2025Article
- Fertility restorer geneHorticulture research · 2024Article
- Fine mapping of Rf2, a minor Restorer-of-fertility (Rf) gene for cytoplasmic male sterility in chili pepper G164 (Capsicum annuum L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Development and application of KASP markers associated withPhysiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Genomic Scan of Male Fertility Restoration Genes in a 'Gülzow' Type Hybrid Breeding System of Rye (International journal of molecular sciences · 2021Article
- Genetic mapping of pollen fertility restoration QTLs in rye (Secale cereale L.) with CMS Pampa.Journal of applied genetics · 2021Article
- Fine mapping of the male fertility restoration gene CaRf032 in Capsicum annuum L.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Article
- Transcriptomics, chromosome engineering and mapping identify a restorer-of-fertility region in the CMS wheat system msH1.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Article
- Undesired fertility restoration in msm1 barley associates with two mTERF genes.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Article
- High intraspecific diversity of Restorer-of-fertility-like genes in barley.The Plant journal : for cell and molecular biology · 2019Article
- Fine mapping of the restorer gene Rfp3 from an Iranian primitive rye (Secale cereale L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2017Article
- In Silico Identification of Candidate Genes for Fertility Restoration in Cytoplasmic Male Sterile Perennial Ryegrass (Lolium perenne L.).Genome biology and evolution · 2017Article
- Evolutionary plasticity of restorer-of-fertility-like proteins in rice.Scientific reports · 2016Article
- Comparison of SNP and CAPS markers application in genetic research in wheat and barley.BMC plant biology · 2016Review
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8 authors.
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Abstract
key messageHigh-resolution genetic linkage mapping and BAC physical mapping narrowed the fertility restorer locus Rfm1 in barley to a sub-centimorgan genetic interval and a 208-kb physical interval. Rfm1 restores the fertility of msm1 and msm2 male-sterile cytoplasms in barley. The fertility restoration gene is located on the short arm of chromosome 6H (6HS), and we pursued a positional cloning of this gene. Starting from a previous result that has delimited Rfm1 within a 10.8 cM region on 6HS, we developed novel CAPS and SSR markers tightly linked to the gene in barley using the sequence information from the syntenic region of rice and barley genome assemblies. Next, we performed fine mapping of the Rfm1 locus. To isolate recombinants, we surveyed 3,638 F2 plants derived from a cross between the CMS strain and the Rf strain with adjacent markers (NAS2090 and NAS1080). This analysis identified 175 recombinant plants from the F2 population to build a high-resolution map with nine markers tightly linked to the Rfm1 locus. Rfm1 was located within the 0.14 cM region delimited by two markers (NAS9113 and NAS9200). Using these flanking markers as well as marker cosegregating with Rfm1 (NAS9133), we screened the BAC libraries of the cultivar Morex, an rfm1 carrier. We isolated 11 BAC clones and constructed a BAC physical map using their fingerprints. Finally, we delimited the Rfm1 locus encompassing the rfm1 allele on a 208-kb contig composed of three minimally overlapping BAC clones. This precise localization of the Rfm1 locus in the barley genome is expected to greatly accelerate the future map-based cloning of the Rfm1 gene by sequence analysis and its genetic transformation for the complementation of cytoplasmic male-sterile plants.
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