Evidence map›Paper›PMID 25412619›Full record

ArticleToxicological sciences : an official journal of the Society of Toxicology2015

Carbon nanotubes induce apoptosis resistance of human lung epithelial cells through FLICE-inhibitory protein.

Varisa Pongrakhananon, Sudjit Luanpitpong, Todd A Stueckle, Liying Wang, Ubonthip Nimmannit, Yon Rojanasakul

Registry-linked trialOpen access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Toxicological sciences : an official journal of the Society of Toxicology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT03300167 (Study of the Vascular Response to Percutaneous Coronary Intervention in Patients With Non-ST-elevation Acute Coronary Syndromes Using Intravascular Blood Sampling), which is not on this map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.0field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT03300167 nawithdrawnnot on this mapstarted 2017, after this paper: background citation

Study of the Vascular Response to Percutaneous Coronary Intervention in Patients With Non-ST-elevation Acute Coronary Syndromes Using Intravascular Blood Sampling

TypeinterventionalSponsorSheffield Teaching Hospitals NHS Foundation TrustRan2017 to 2020Enrolled0ConditionsNon ST Segment Elevation Acute Coronary SyndromeArmsThe Liquid Biopsy System
3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
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  3. Review
  4. Article
  5. Review
  6. Article
  7. Article
  8. Article
  9. Role of mesothelin in carbon nanotube-induced carcinogenic transformation of human bronchial epithelial cells.American journal of physiology. Lung cellular and molecular physiology · 2016
    Article
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 2 countries.

Varisa Pongrakhananon*Department of Pharmaceutical Sciences, West Virginia University, Morgantown, West Virginia 26506, Department of Pharmacology and Physiology, Chulalongkorn University, Bangkok, Thailand, Mary Babb Randolph Cancer Center, West Virginia University, Morgantown, West Virginia 26506, Siriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand, Allergy and Clinical Immunology Branch, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505, and National Nanotechnology Center, Pathumthani, Thailand *Department of Pharmaceutical Sciences, West Virginia University, Morgantown, West Virginia 26506, Department of Pharmacology and Physiology, Chulalongkorn University, Bangkok, Thailand, Mary Babb Randolph Cancer Center, West Virginia University, Morgantown, West Virginia 26506, Siriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand, Allergy and Clinical Immunology Branch, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505, and National Nanotechnology Center, Pathumthani, Thailand.
Sudjit Luanpitpong*Department of Pharmaceutical Sciences, West Virginia University, Morgantown, West Virginia 26506, Department of Pharmacology and Physiology, Chulalongkorn University, Bangkok, Thailand, Mary Babb Randolph Cancer Center, West Virginia University, Morgantown, West Virginia 26506, Siriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand, Allergy and Clinical Immunology Branch, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505, and National Nanotechnology Center, Pathumthani, Thailand *Department of Pharmaceutical Sciences, West Virginia University, Morgantown, West Virginia 26506, Department of Pharmacology and Physiology, Chulalongkorn University, Bangkok, Thailand, Mary Babb Randolph Cancer Center, West Virginia University, Morgantown, West Virginia 26506, Siriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand, Allergy and Clinical Immunology Branch, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505, and National Nanotechnology Center, Pathumthani, Thailand *Department of Pharmaceutical Sciences, West Virginia University, Morgantown, West Virginia 26506, Department of Pharmacology and Physiology, Chulalongkorn University, Bangkok, Thailand, Mary Babb Randolph Cancer Center, West Virginia University, Morgantown, West Virginia 26506, Siriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand, Allergy and Clinical Immunology Branch, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505, and National Nanotechnology Center, Pathumthani, Thailand.
Todd A Stueckle*Department of Pharmaceutical Sciences, West Virginia University, Morgantown, West Virginia 26506, Department of Pharmacology and Physiology, Chulalongkorn University, Bangkok, Thailand, Mary Babb Randolph Cancer Center, West Virginia University, Morgantown, West Virginia 26506, Siriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand, Allergy and Clinical Immunology Branch, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505, and National Nanotechnology Center, Pathumthani, Thailand *Department of Pharmaceutical Sciences, West Virginia University, Morgantown, West Virginia 26506, Department of Pharmacology and Physiology, Chulalongkorn University, Bangkok, Thailand, Mary Babb Randolph Cancer Center, West Virginia University, Morgantown, West Virginia 26506, Siriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand, Allergy and Clinical Immunology Branch, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505, and National Nanotechnology Center, Pathumthani, Thailand.
Liying Wang*Department of Pharmaceutical Sciences, West Virginia University, Morgantown, West Virginia 26506, Department of Pharmacology and Physiology, Chulalongkorn University, Bangkok, Thailand, Mary Babb Randolph Cancer Center, West Virginia University, Morgantown, West Virginia 26506, Siriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand, Allergy and Clinical Immunology Branch, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505, and National Nanotechnology Center, Pathumthani, Thailand.
Ubonthip Nimmannit*Department of Pharmaceutical Sciences, West Virginia University, Morgantown, West Virginia 26506, Department of Pharmacology and Physiology, Chulalongkorn University, Bangkok, Thailand, Mary Babb Randolph Cancer Center, West Virginia University, Morgantown, West Virginia 26506, Siriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand, Allergy and Clinical Immunology Branch, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505, and National Nanotechnology Center, Pathumthani, Thailand.
Yon Rojanasakul*Department of Pharmaceutical Sciences, West Virginia University, Morgantown, West Virginia 26506, Department of Pharmacology and Physiology, Chulalongkorn University, Bangkok, Thailand, Mary Babb Randolph Cancer Center, West Virginia University, Morgantown, West Virginia 26506, Siriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand, Allergy and Clinical Immunology Branch, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505, and National Nanotechnology Center, Pathumthani, Thailand *Department of Pharmaceutical Sciences, West Virginia University, Morgantown, West Virginia 26506, Department of Pharmacology and Physiology, Chulalongkorn University, Bangkok, Thailand, Mary Babb Randolph Cancer Center, West Virginia University, Morgantown, West Virginia 26506, Siriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand, Allergy and Clinical Immunology Branch, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505, and National Nanotechnology Center, Pathumthani, Thailand yrojan@hsc.wvu.edu.
Mahidol University · TH

Funding

West Virginia Flow Cytometry Core FacilityP30GM103488 · NIGMS · WEST VIRGINIA UNIVERSITY · PI GIBSON, LAURA F. · 2012 to 2016
$4.8M
Induction of Neoplastic Transformation and Cancer Stem Cells by Carbon NanotubesR01ES022968 · NIEHS · WEST VIRGINIA UNIVERSITY · PI ROJANASAKUL, YON · 2014 to 2019
$1.7M
Prediction and Mechanism of Carbon Nanotube-Induced FibrosisR01HL095579 · NHLBI · WEST VIRGINIA UNIVERSITY · PI ROJANASAKUL, YON · 2010 to 2013
$1.5M
NHLBI NIH HHS R01-HL095579NIEHS NIH HHS R01 ES022968NIEHS NIH HHS R01-ES022968NIGMS NIH HHS P30 GM103488
6 · The paper itself

Abstract

Chronic exposure to single-walled carbon nanotubes (SWCNT) has been reported to induce apoptosis resistance of human lung epithelial cells. As resistance to apoptosis is a foundation of neoplastic transformation and cancer development, we evaluated the apoptosis resistance characteristic of the exposed lung cells to understand the pathogenesis mechanism. Passage control and SWCNT-transformed human lung epithelial cells were treated with known inducers of apoptosis via the intrinsic (antimycin A and CDDP) or extrinsic (FasL and TNF-α) pathway and analyzed for apoptosis by DNA fragmentation, annexin-V expression, and caspase activation assays. Whole-genome microarray was performed to aid the analysis of apoptotic gene signaling network. The SWCNT-transformed cells exhibited defective death receptor pathway in association with cellular FLICE-inhibitory protein (c-FLIP) overexpression. Knockdown or chemical inhibition of c-FLIP abrogated the apoptosis resistance of SWCNT-transformed cells. Whole-genome expression signature analysis confirmed these findings. This study is the first to demonstrate carbon nanotube-induced defective death receptor pathway and the role of c-FLIP in the process.

Indexed as

ApoptosisCASP8 and FADD-Like Apoptosis Regulating ProteinCell Culture TechniquesCell LineCell Transformation, NeoplasticDose-Response Relationship, DrugDrug ResistanceEpithelial CellsGene Knockdown TechniquesHumansLungNanotubes, CarbonReceptors, Death DomainCASP8 and FADD-Like Apoptosis Regulating ProteinNanotubes, CarbonReceptors, Death Domainapoptosiscarbon nanotubesc-FLIPdeath receptorlung

Identifiers

PMID25412619
PMCPMC4306727
OpenAlexW2023084993

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Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.